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dc.contributor.authorCampuzano, Susana-
dc.contributor.authorEsteban-Fernández de Ávila, Berta-
dc.contributor.authorYuste, José-
dc.contributor.authorPedrero, María-
dc.contributor.authorGarcía, José Luis-
dc.contributor.authorGarcía, Pedro-
dc.contributor.authorGarcía, Ernesto-
dc.contributor.authorPingarrón, José Manuel-
dc.identifier.citationBiosens Bioelectron 26(4):1225-1230(2010)es_ES
dc.description6 páginas, 4 figuras -- PAGS nros. 1225-1230es_ES
dc.description.abstractDisposable amperometric magnetoimmunosensors, based on the use of functionalized magnetic beads and gold screen-printed electrodes, have been developed for the selective detection and quantification of Streptococcus pneumoniae. A specific antibody prepared against a serotype 37 S. pneumoniae strain, selected by flow cytometry among seven anticapsular or antisomatic antibodies, was linked to Protein A-modified magnetic beads and incubated with bacteria. The same antibody, conjugated with horseradish peroxidase, was attached to the bacteria and the resulting modified magnetic beads were captured by a magnetic field on the surface of tetrathiafulvalene-modified gold screen-printed electrodes. The amperometric response obtained at −0.15 V vs. the silver pseudoreference electrode of the Au/SPE after the addition of H2O2 was used as transduction signal. Different assay formats were examined and the experimental variables optimized. The limits of detection achieved, without pre-concentration or pre-enrichment steps, were 1.5 × 104 cfu mL−1 (colony forming unit) and 6.3 × 105 cfu mL−1 for S. pneumoniae strains Dawn (serotype 37) and R6 (non-encapsulated), respectively. The developed methodology shows a good selectivity against closely related streptococci and its usefulness for the analysis of inoculated urine samples has been demonstrated. The total analysis time of 3.5 h from sampling to measurement, the possibility to prepare up to 30 sensors per day and the use of small amounts of test solution for S. pneumoniae identification, constitute important advantages that make the developed methodology a promising alternative for clinical diagnosises_ES
dc.description.sponsorshipThis research was supported by grants from the Dirección General de Investigación Científica y Técnica (SAF2006-00390 and SAF2009-10824). The financial support of Santander/Complutense Research Project PR 27/05-13953, the Spanish Ministerio de Ciencia e Innovación Research Project CTQ2009-09351BQU, and the AVANSENS Programme from the Comunidad de Madrid (S2009PPQ-1642) are also gratefully acknowledged. S. Campuzano acknowledges a “Juan de la Cierva” research contract to the Spanish Ministerio de Ciencia e Innovaciónes_ES
dc.subjectS. pneumoniaees_ES
dc.subjectMagnetic beadses_ES
dc.subjectGold screen-printed electrodeses_ES
dc.titleDisposable amperometric magnetoimmunosensors for the specific detection of Streptococcus pneumoniaees_ES
dc.description.peerreviewedPeer reviewedes_ES
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