English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/52368
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Replication fork breakage and re-start: New insights into Rad3/XPD-associated deficiencies

AuthorsMoriel-Carretero, María ; Aguilera, Andrés
KeywordsRad3/XPD
TFIIH
Replication fork breakage and re-star
Rad51
Pol32
Nucleotide excision repair
Double-strand break repair
Homologous recombination
Xeroderma pigmentosum
Cockayne syndrome
Issue Date1-Aug-2010
PublisherLandes Bioscience
CitationCell Cycle 9(15): 2958-2962 (2010)
AbstractFailures in Nucleotide Excision Repair (NER) are generally associated with extreme sun sensitivity, high cancer risks and neurodegeneration. This is explained by the inability to repair UV lesions and oxidative damage, and may be ascribed to a deficiency in the TFIIH complex, which has a dual role in NER and transcription initiation. We have recently uncovered the molecular basis for a specific TFIIH component deficiency, Rad3/XPD, whose consequences are drastically different from other NER failures. Yeast rad3-102 cells partially process NER damage beyond the incision step but do not refill the generated ssDNA gap, as a consequence leading to replication fork breakage. Double-strand breaks are therefore generated that need to be repaired by a Rad52 and MRX-dependent homologous recombination mechanism, which promotes replication re-start via two alternative pathways, one Rad51-dependent, the other Pol32-dependent. On the basis of this study we revisit and discuss our actual view of replication fork breakage and re-start and the molecular mechanisms that explain XPD-associated diseases.
URIhttp://hdl.handle.net/10261/52368
DOI10.4161/cc.9.15.12408
Identifiersissn: 1538-4101
Appears in Collections:(CABIMER) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.