English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/52180
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Chimeric tRNAs as tools to induce proteome damage and identify components of stress responses

AuthorsGeslain, Renaud; Cubells, Laia; Bori-Sanz, Teresa; Álvarez-Medina, Roberto ; Rossell, David; Martí, Elisa ; Ribas de Pouplana, Lluís
Issue Date2010
PublisherOxford University Press
CitationNucleic Acids Research 38(5): e30 (2010)
AbstractMisfolded proteins are caused by genomic mutations, aberrant splicing events, translation errors or environmental factors. The accumulation of misfolded proteins is a phenomenon connected to several human disorders, and is managed by stress responses specific to the cellular compartments being affected. In wild-type cells these mechanisms of stress response can be experimentally induced by expressing recombinant misfolded proteins or by incubating cells with large concentrations of amino acid analogues. Here, we report a novel approach for the induction of stress responses to protein aggregation. Our method is based on engineered transfer RNAs that can be expressed in cells or tissues, where they actively integrate in the translation machinery causing general proteome substitutions. This strategy allows for the introduction of mutations of increasing severity randomly in the proteome, without exposing cells to unnatural compounds. Here, we show that this approach can be used for the differential activation of the stress response in the Endoplasmic Reticulum (ER). As an example of the applications of this method, we have applied it to the identification of human microRNAs activated or repressed during unfolded protein stress. © The Author(s) 2009. Published by Oxford University Press.
DescriptionThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License.
Publisher version (URL)http://dx.doi.org/10.1093/nar/gkp1083
Identifiersdoi: 10.1093/nar/gkp1083
issn: 0305-1048
e-issn: 1362-4962
Appears in Collections:(IBMB) Artículos
Files in This Item:
File Description SizeFormat 
ChimericTRNAsAsToolsToInduce_Geslain.pdf6,84 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.