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Título: | Optimization of extraction and purification process of hyaluronic acid from fish eyeball |
Autor: | Murado García, Miguel Anxo; Montemayor, María I. CSIC; López Cabo, Marta CSIC ORCID; Vázquez, José Antonio CSIC ORCID; González Fernández, Pilar CSIC | Palabras clave: | Hyaluronic acid Fish by-products Bioprocessing Environment Food processing Downstream processing |
Fecha de publicación: | 2012 | Editor: | Elsevier | Citación: | Food and Bioproducts Processing 90(3): 491-498 (2012) | Resumen: | The goal of the present work is to optimize the different steps for obtaining highly purified hyaluronic acid (HA) from fish eyeball. The extraction and purification process of HA from vitreous humour of fish, among other biological materials, is based on the succession of: (1) a step of protein electrodeposition, previous or simultaneous with a diafiltration process in total recirculation, (2) a selective recovery in hydroalcoholic solution of impure sediments obtained by alcoholic exhaustive precipitation, (3) an alkaline treatment under hydroalcoholic solution and controlled conditions of alkalinity, temperature, proportion of ethanol and time that it precipitates HA and solubilises proteins, and (4) HA recovery by alkaline suspension of the precipitate in hydroalcoholic phosphate monosodium that it dissolves HA, neutralizes the extract and leaves insoluble proteins in the sediment. Thus, HA with high purity (more than 99.5%), useful for clinical and cosmetic applications, are obtained by means of low-cost process using a waste material. | Descripción: | 8 páginas, 3 tablas, 4 figuras | Versión del editor: | http://dx.doi.org/10.1016/j.fbp.2011.11.002 | URI: | http://hdl.handle.net/10261/52179 | DOI: | 10.1016/j.fbp.2011.11.002 | ISSN: | 0960-3085 |
Aparece en las colecciones: | (IIM) Artículos |
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Optimization_extraction_purification.pdf | 256,54 kB | Adobe PDF | Visualizar/Abrir |
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