English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/52044
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Lumican is down-regulated in cells expressing endoglin. Evidence for an inverse correlationship between endoglin and lumican expression

AuthorsBotella, Luisa María ; Sánz-Rodríguez, Francisco; Sánchez-Elsner, Tilman; Langa, Carmen ; Ramírez, José Ramón; Vary, Calvin P. H.; Roughley, Peter J.; Bernabéu, Carmelo
SLR proteoglycans
Extracellular matrix
Differential expression
Issue DateJan-2004
CitationMatrix Biology 22(7):561-572(2004)
AbstractEndoglin (CD105) is a homodimeric membrane glycoprotein, which acts as a TGF-β coreceptor in the vasculature and plays an important role in cardiovascular development and vascular remodelling. To isolate putative genes regulated by endoglin expression, a PCR-based RNA fingerprinting technique was carried out. Myoblasts stably transfected with endoglin showed a decrease in the expression of lumican both at the RNA and protein levels. Lumican is a proteoglycan of the extracellular matrix, belonging to the SLRP (Small Leucine-Rich Repeat Proteoglycans) family. Lumican down-regulation by endoglin appeared to be controlled, at least in part, at the transcriptional level, as indicated by RT-PCR, and transient transfection experiments using a lumican promoter reporter based vector. This inverse correlation between endoglin and lumican expression was substantiated by immunohistochemical staining of vessels from human tissues. Thus, cells belonging to the high endothelia, such as tonsil, express a large amount of endoglin, and the lumican content of their matrix is considerably reduced. Conversely, in resting endothelia, such as that of large vessels, the expression of endoglin is reduced whereas the amount of lumican is greatly increased. The inverse regulation in the expression of endoglin and lumican was also evident after TGF-β treatments since endoglin was up-regulated, whereas lumican was down-regulated by this cytokine. This report describes for the first time a relationship between endoglin and lumican expression
Description12 páginas, 6 figuras, 1 tabla -- PAGS nros. 561-572
Publisher version (URL)http://dx.doi.org/10.1016/j.matbio.2003.11.006
Appears in Collections:(CIB) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.