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Título : Quantitative detection of clostridium tyrobutyricum in milk by real-time PCR
Autor : López-Enríquez, Lorena; Rodríguez-Lázaro, David; Hernández, Marta
Fecha de publicación : jun-2007
Editor: American Society for Microbiology
Citación : Applied and Environmental Microbiology 73(11): 3747–3751 (2007)
Resumen: We developed a real-time PCR assay for the quantitative detection of Clostridium tyrobutyricum, which has been identified as the major causal agent of late blowing in cheese. The assay was 100% specific, with an analytical sensitivity of 1 genome equivalent in 40% of the reactions. The quantification was linear (R2 > 0.9995) over a 5-log dynamic range, down to 10 genome equivalents, with a PCR efficiency of >0.946. With optimized detergent treatment and enzymatic pretreatment of the sample before centrifugation and nucleic acid extraction, the assay counted down to 300 C. tyrobutyricum spores, with a relative accuracy of 82.98 to 107.68, and detected as few as 25 spores in 25 ml of artificially contaminated raw or ultrahigh-temperature-treated whole milk.
Versión del editor: http://dx.doi.org/10.1128/AEM.02642-06
URI : http://hdl.handle.net/10261/5200
DOI: 10.1128/AEM.02642-06
ISSN: 0099-2240
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