Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/5199
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Modulation of Ca2+ release and Ca2+ oscillations in HeLa cells and fibroblasts by mitochondrial Ca2+ uniporter stimulation

AuthorsVay, Laura; Hernández-SanMiguel, Esther; Santo-Domingo, Jaime; Lobatón, Carmen D.; Moreno, Alfredo; Montero, Mayte CSIC ORCID CVN; Álvarez, Javier
Issue Date1-Apr-2007
PublisherBlackwell Publishing
CitationJ Physiol. 2007 April 1; 580(Pt 1): 39–49
AbstractThe recent availability of activators of the mitochondrial Ca2+ uniporter allows direct testing of the influence of mitochondrial Ca2+ uptake on the overall Ca2+ homeostasis of the cell. We show here that activation of mitochondrial Ca2+ uptake by 4,4′,4″-(4-propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT) or kaempferol stimulates histamine-induced Ca2+ release from the endoplasmic reticulum (ER) and that this effect is enhanced if the mitochondrial Na+–Ca2+ exchanger is simultaneously inhibited with CGP37157. This suggests that both Ca2+ uptake and release from mitochondria control the ability of local Ca2+ microdomains to produce feedback inhibition of inositol 1,4,5-trisphosphate receptors (InsP3Rs). In addition, the ability of mitochondria to control Ca2+ release from the ER allows them to modulate cytosolic Ca2+ oscillations. In histamine stimulated HeLa cells and human fibroblasts, both PPT and kaempferol initially stimulated and later inhibited oscillations, although kaempferol usually induced a more prolonged period of stimulation. Both compounds were also able to induce the generation of Ca2+ oscillations in previously silent fibroblasts. Our data suggest that cytosolic Ca2+ oscillations are exquisitely sensitive to the rates of mitochondrial Ca2+ uptake and release, which precisely control the size of the local Ca2+ microdomains around InsP3Rs and thus the ability to produce feedback activation or inhibition of Ca2+ release.
DescriptionCopyright © by Blackwell Publishing Ltd.-- The definitive version is available at www.blackwell-synergy.com
URIhttp://hdl.handle.net/10261/5199
DOI10.1113/jphysiol.2006.126391
ISSN0022-3751
Appears in Collections:(IBGM) Artículos

Show full item record
Review this work

PubMed Central
Citations

21
checked on May 17, 2022

SCOPUSTM   
Citations

42
checked on May 17, 2022

WEB OF SCIENCETM
Citations

38
checked on May 12, 2022

Page view(s)

332
checked on May 17, 2022

Google ScholarTM

Check

Altmetric

Dimensions


Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.