English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/51930
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Molecular and cytological characterization of repetitive DNA sequences from the centromeric heterochromatin of Sciara coprophila

AuthorsEscribá Pérez, M. Carmen; Greciano, Patricia G. ; Méndez-Lago, María ; Pablos, Beatriz de ; Trifonov, Vladimir A.; Ferguson-Smith, Malcolm A.; Goday, Clara ; Villasante, Alfredo
Issue DateAug-2011
CitationChromosoma 120(4):387-397(2011)
AbstractSciara coprophila (Diptera, Nematocera) constitutes a classic model to analyze unusual chromosome behavior such as the somatic elimination of paternal X chromosomes, the elimination of the whole paternal, plus non-disjunction of the maternal X chromosome at male meiosis. The molecular organization of the heterochromatin in S. coprophila is mostly unknown except for the ribosomal DNA located in the X chromosome pericentromeric heterochromatin. The characterization of the centromeric regions, thus, is an essential and required step for the establishment of S. coprophila as a model system to study fundamental mechanisms of chromosome segregation. To accomplish such a study, heterochromatic sections of the X chromosome centromeric region from salivary glands polytene chromosomes were microdissected and microcloned. Here, we report the identification and characterization of two tandem repeated DNA sequences from the pericentromeric region of the X chromosome, a pericentromeric RTE element and an AT-rich centromeric satellite. These sequences will be important tools for the cloning of S. coprophila centromeric heterochromatin using libraries of large genomic clones
Description11 páginas, 8 figuras -- PAGS nros. 387-397
Publisher version (URL)http:dx.doi.org/10.1007/s00412-011-0320-2
Appears in Collections:(CIB) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.