Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/51838
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Guasch, Alicia | - |
dc.contributor.author | Lucas, María | - |
dc.contributor.author | Moncalián, Gabriel | - |
dc.contributor.author | Cabezas, Matilde | - |
dc.contributor.author | Pérez-Luque, Rosa | - |
dc.contributor.author | Gomis-Rüth, F. Xavier | - |
dc.contributor.author | Cruz, Fernando de la | - |
dc.contributor.author | Coll, Miquel | - |
dc.date.accessioned | 2012-06-19T10:28:41Z | - |
dc.date.available | 2012-06-19T10:28:41Z | - |
dc.date.issued | 2003 | - |
dc.identifier | doi: 10.1038/nsb1017 | - |
dc.identifier | issn: 1545-9993 | - |
dc.identifier.citation | Nature Structural and Molecular Biology 10(12): 1002-1010 (2003) | - |
dc.identifier.uri | http://hdl.handle.net/10261/51838 | - |
dc.description.abstract | Relaxases are DNA strand transferases that catalyze the initial and final stages of DNA processing during conjugative cell-to-cell DNA transfer. Upon binding to the origin of transfer (oriT) DNA, relaxase TrwC melts the double helix. The three-dimensional structure of the relaxase domain of TrwC in complex with its cognate DNA at oriT shows a fold built on a two-layer α/β sandwich, with a deep narrow cleft that houses the active site. The DNA includes one arm of an extruded cruciform, an essential feature for specific recognition. This arm is firmly embraced by the protein through a β-ribbon positioned in the DNA major groove and a loop occupying the minor groove. It is followed by a single-stranded DNA segment that enters the active site, after a sharp U-turn forming a hydrophobic cage that traps the N-terminal methionine. Structural analysis combined with site-directed mutagenesis defines the architecture of the active site. | - |
dc.description.sponsorship | This study was supported by the Spain Ministerio de Educación y Ciencia, the Generalitat de Catalunya and the European Union (EU). Synchrotron data collection was supported by the European Synchrotron Radiation Facility (ESRF) and the EU. | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.rights | closedAccess | - |
dc.title | Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1038/nsb1017 | - |
dc.date.updated | 2012-06-19T10:28:41Z | - |
dc.description.version | Peer Reviewed | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (IBBTEC) Artículos (IBMB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
123
checked on 21-abr-2024
WEB OF SCIENCETM
Citations
121
checked on 24-feb-2024
Page view(s)
398
checked on 23-abr-2024
Download(s)
120
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.