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Title

Role of peptide lunasin's antioxidant activity on its chemopreventive properties

AuthorsHernández-Ledesma, Blanca ; Espartosa, D.; Hsieh, Chia-Chien; Lumen, Ben O. de; Recio, Isidra
KeywordsLunasin
Antioxidant activity
Macrophages
Issue Date2011
CitationFENS 2011
Abstract[Introduction]: Oxidative stress and inflammation are two of the most critical factors implicated in carcinogenesis and other degenerative disorders. Consequently, determination of anti-inflammatory and/or antioxidant properties has been proposed as a good indicator for screening anti-cancer agents. Lunasin is a peptide identified in soybean and other plants which properties against cancer have been demonstrated in both in vitro assays and in vivo mouse models. Different mechanisms of action have been revealed as relevant on lunasin’s chemopreventive role, including inhibition of cell proliferation, arrest of cell cycle, apoptosis induction, and modulation of genes and protein expression involved in different carcinogenesis pathways. Preliminary studies have demonstrated lunasin’s activity as antiinflammatory food peptide. However, no data about its antioxidant activity in both in vitro and cell culture experiments have reported to date. [Objectives]: The main aim of this work was to evaluate the contribution of lunasin’s antioxidant activity on its chemopreventive properties. [Method/Design]: Initially, different in vitro assays were performed to evaluate lunasin’s mechanism of antioxidant action. The capacity of this peptide to scavenge radicals and iron, as well as its ability to reduce Fe3+ to Fe2+, and to inhibit linoleic acid oxidation was analyzed. Moreover, macrophages RAW 264.7 cells and intestinal cells were used to determine lunasin’s effect in the production of intracellular oxygen radicals species (ROS) as well as in glutathione (GSH) levels. [Results]: Lunasin demonstrated to exert potent radicals scavenging activity, showing also a moderate capacity to scanvenge iron. Studies performed in RAW 264.7 cells showed that this food peptide does not affect cell viability, reducing ROS generation. Preliminary results indicate a similar inhibitory effect of ROS production in Caco2 cells. [Conclusions]: This study demonstrates that lunasin exerts a notable antioxidant activity through different mechanisms of action, protecting mammalian cells against oxidant compounds produced in excess during metabolic process. This activity might contribute on lunasin’s chemopreventive role against cancer and other oxidative stress-related disorders.
DescriptionResumen del póster presentado al 11th European Nutrition Conference celebrado en Madrid del 26 al 29 de octubre de 2011.
URIhttp://hdl.handle.net/10261/51822
Appears in Collections:(CIAL) Comunicaciones congresos
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