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Immobilization of a recombinant endo-1,5-arabinanase secreted by Aspergillus nidulans strain A773

AutorPessela, Benevides C. ; Mateo González, César ; Guisán, José Manuel ; Teixeira de Moraes Polizeli, Maria de Lourdes
Fecha de publicación2012
EditorElsevier
CitaciónJournal of Molecular Catalysis B: Enzymatic 77: 39-45 (2012)
ResumenAn endo-1,5-arabinanase (abnA) encoding gene from Aspergillus niveus was identified, cloned and successfully expressed in Aspergillus nidulans strain A773. Based on amino acid sequence comparison, the 34-kDa enzyme could be assigned to CAZy GH family 43. Characterization of purified recombinant endo-1,5-arabinanase (AbnA) revealed that it is active at a wide pH range (pH 4.0-7.0) and an optimum temperature at 70 °C. The immobilization of the AbnA was performed via covalent binding onto agarose-modified supports: glyoxyl iminodiacetic acid-Ni 2+, glyoxyl amine, glyoxyl (4% and 10%) and cyanogen bromide activated sepharose. The yield of immobilization was similar on glyoxyl amine and glyoxyl (96%), and higher than glyoxyl iminodiacetic acid-Ni 2+ (43%) support. The thermal inactivation of these immobilized preparations showed that the stability of the AbnA immobilized on glyoxyl 4 and 10% was improved by 4.0 and 10.3-fold factor at 70 °C. The half-life of glyoxyl 4% derivative at 60 °C was >48 h (pH 5), 9 h (pH 7) and 88 min (pH 9). The major hydrolysis product of debranched arabinan or arabinopentaose by glyoxyl agarose-immobilized AbnA was arabinobiose. © 2012 Elsevier B.V. All rights reserved.
URIhttp://hdl.handle.net/10261/51724
DOI10.1016/j.molcatb.2012.01.002
Identificadoresdoi: 10.1016/j.molcatb.2012.01.002
issn: 1381-1177
e-issn: 1873-3158
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