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Food derived peptides stimulate mucin secretion and gene expression in intestinal cells

AutorRecio, Isidra ; Martínez-Maqueda, D. ; Miralles, Beatriz ; Pascual-Teresa, Sonia de ; Reverón, Inés ; Muñoz, Rosario ; Ramos, Mercedes
Palabras claveGene expression
Opioid peptides
Intestinal cells
Fecha de publicación2011
EditorS. Karger AG
CitaciónAnnals of Nutrition and Metabolism 58(suppl 3): 117 (2011)
Resumen[Introduction]: Certain dietary components, such as fiber, probiotics, etc, can positively influence characteristics of the intestinal mucus, although the mode of action of each compound may differ. Recently it has been demonstrated that specific peptide sequences, concretely β-casomorphin 7, induces a strong release of mucin the jejunum of the rat through the activation of the enteric nervous system and opioid receptors. The presence of opioid receptors on intestinal cells suggests the possibility that food-derived opioid peptides, which can be produced in the intestinal lumen during gastrointestinal digestion, might modulate the production of mucin via a direct action on epithelial goblet cells.
[Objectives]: The aim of this work was to evaluate the mucin secretion and MUC5AC expression induced by food-derived opioid peptides on intestinal cells.
[Method/Design]: Different opioid food derived peptides with ability to bind mu or delta opioid receptors were tested on HT29-MTX human colonic cells seeded until confluency. An enzyme-linked lectin assay (ELLA) was used to measure mucinlike glycoprotein secretion. In those peptides showing significant higher secretion, quantitative PCR was used to determine the level of transcripts.
[Results]: HT29-MTX human colonic cells exhibited increased secretion of mucin over time and therefore proved to be a reliable tool for the study of regulation of gastrointestinal mucin expression and secretion. Significantly increased secretion of mucin after 2 or 4 hours after the exposure to some peptides was found. Among them, α-lactorfin, a peptide derived from α-lactalbumin, showed increased expression of MUC5AC.
[Conclusions]: Milk opioid peptides have shown to induce mucin secretion and regulate the expression of MUC5AC in HT29-MTX human colonic gobletlike cells. Milk opioid-derived peptides could thus provide new prospects for improving gastrointestinal protection.
DescripciónResumen del trabajo presentado a la "11th European Nutrition Conference (FENS)" celebrada en Madrid del 26 al 29 de octubre de 2011.
Versión del editorhttp://dx.doi.org/10.1159/000334393
Identificadoresissn: 0250-6807
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