English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/51707
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Feasibility and application of liquid-liquid extraction combined with gas chromatography-mass spectrometry for the analysis of phenolic acids from grape polyphenols degraded by human faecal microbiota

AuthorsMuñoz-González, Carolina ; Moreno-Arribas, M. Victoria ; Rodríguez-Bencomo, Juan José ; Cueva, Carolina ; Martín-Álvarez, Pedro J. ; Bartolomé, Begoña ; Pozo-Bayón, Mª Ángeles
Issue Date2012
CitationFood Chemistry 133(2): 526-535 (2012)
AbstractIn this study the feasibility of a LLE-GC-EI-MS method for the analysis of 43 phenolic acids belonging to different chemical structure families which have been described in the literature as microbial-derived metabolites after consumption of dietary polyphenols was proved. In addition, the method was applied for the characterisation of phenolic metabolites resulting from the incubation, in anaerobic conditions, of a commercial grape seed extract (GSE) and their corresponding flavan-3-ol monomeric (GSE-M) and oligomeric (GSE-O) fractions with human faeces from healthy volunteers (n = 3). The method showed average values of repeatability and reproducibility of 5.0% and 6.3%, respectively, adequate and low detection (1.8-30.8 μg L -1) and quantification limits (6.0-102.8 μg L -1) and good recovery values (95%, as average value). A total of 27 phenolic acids were identified in the faecal solutions after incubation with the grape seed extracts. In general, faecal samples incubated with GSE and GSE-M (monomeric fraction) yield a higher formation of phenolic acids compared to the samples incubated with the oligomer fraction (GSE-O). © 2012 Elsevier Ltd. All rights reserved.
Publisher version (URL)http://dx.doi.org/10.1016/j.foodchem.2012.01.020
Identifiersdoi: 10.1016/j.foodchem.2012.01.020
issn: 0308-8146
Appears in Collections:(CIAL) Artículos
Files in This Item:
File Description SizeFormat 
Feasibility and application.pdf143,08 kBAdobe PDFThumbnail
Figure 1.pdf71,32 kBAdobe PDFThumbnail
Table 1.pdf35,08 kBAdobe PDFThumbnail
Table 2.pdf33,84 kBAdobe PDFThumbnail
Table 3.pdf70,61 kBAdobe PDFThumbnail
Table 4.pdf91,34 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.