Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/5169
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Coralyne cation, a fluorescent probe for general detection in planar chromatography |
Autor: | Cebolla, Vicente L. CSIC ORCID CVN ; Mateos Serrano, Elena CSIC; Membrado, Luis CSIC ORCID; Vela, Jesús CSIC ORCID; Gálvez Buerba, Eva Mª CSIC ORCID ; Matt, Muriel; Cossío, Fernando P. | Palabras clave: | Detection Fluorescence scanning densitometry Planar chromatography |
Fecha de publicación: | 8-feb-2007 | Editor: | Elsevier BV | Citación: | Journal of Chromatography - A 1146(2): 251-257 (2007) | Resumen: | A large number of analytes, including non-fluorescent ones, can be sensitively detected by fluorescence scanning densitometry using silica gel HPTLC plates impregnated with a solution of coralyne cation. This is carried out by the variation, increase or decrease, that the corresponding analyte induces on native coralyne emission at a given excitation wavelength. A similar phenomenon was previously described for berberine cation, and Reichardt's dye probes. However, the sensitivity of coralyne in HPTLC detection of non-fluorescent, structurally different analytes (e.g., long-chain alkanes, alcohols, alkylbromides, neutral lipids) is superior to that of the above-mentioned probes. In this work, the analytical viability of this phenomenon for HPTLC detection using coralyne as a probe is explored, and fluorescent responses of a number of analytes on the coralyne system are rationalized in the light of a previously proposed model. This establishes that the resulting intensity for a probe in the presence of a given compound can be explained as a balance between radiative (contribution of non-specific interactions) and non-radiative processes (specific interactions), the latter producing fluorescence quenching. Experimental results and proposed model suggest that this phenomenon may be general for practically all kinds of analytes. | Descripción: | 6 Figures, 2 Tables | Versión del editor: | http://dx.doi.org/10.1016/j.chroma.2007.01.138 | URI: | http://hdl.handle.net/10261/5169 | DOI: | 10.1016/j.chroma.2007.01.138 | ISSN: | 0021-9673 |
Aparece en las colecciones: | (ICB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
J. Chromatogr. A 1146 (2007).pdf | 467,02 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
16
checked on 12-abr-2024
WEB OF SCIENCETM
Citations
16
checked on 27-feb-2024
Page view(s)
478
checked on 18-abr-2024
Download(s)
419
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.