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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/5169

Coralyne cation, a fluorescent probe for general detection in planar chromatography

AutorCebolla, Vicente L.; Mateos Serrano, Elena; Membrado, Luis; Vela, Jesús; Gálvez Buerba, Eva Mª ; Matt, Muriel; Cossio, Fernando Pedro
Palabras claveDetection
Fluorescence scanning densitometry
Planar chromatography
Fecha de publicación8-feb-2007
CitaciónJournal of Chromatography A, 1146 (2007) 251–257
ResumenA large number of analytes, including non-fluorescent ones, can be sensitively detected by fluorescence scanning densitometry using silica gel HPTLC plates impregnated with a solution of coralyne cation. This is carried out by the variation, increase or decrease, that the corresponding analyte induces on native coralyne emission at a given excitation wavelength. A similar phenomenon was previously described for berberine cation, and Reichardt's dye probes. However, the sensitivity of coralyne in HPTLC detection of non-fluorescent, structurally different analytes (e.g., long-chain alkanes, alcohols, alkylbromides, neutral lipids) is superior to that of the above-mentioned probes. In this work, the analytical viability of this phenomenon for HPTLC detection using coralyne as a probe is explored, and fluorescent responses of a number of analytes on the coralyne system are rationalized in the light of a previously proposed model. This establishes that the resulting intensity for a probe in the presence of a given compound can be explained as a balance between radiative (contribution of non-specific interactions) and non-radiative processes (specific interactions), the latter producing fluorescence quenching. Experimental results and proposed model suggest that this phenomenon may be general for practically all kinds of analytes
Descripciónjournal page: http://www.elsevier.com/wps/find/journaldescription.cws_home/502688/description#description
Versión del editorhttp://dx.doi.org/10.1016/j.chroma.2007.01.138
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