New microsatellite loci for Narcissus papyraceus (Amarillydaceae) and cross-amplification in other congeneric species

Abstract

The genus Narcissus L. (Amarillydaceae) is well known for its morphological diversity due to the vast amount of within- and among-species floral variation affecting both perianth and sex organs. Both polymorphisms make the genus a good model system to study floral evolution and style polymorphism (Bar- rett et al., 1996; Pérez-Barrales et al., 2006). The genus is cir- cunmediterranean, and the highest diversity is found in the Iberian Peninsula and Northwestern Africa. One of the most common species occurring in the core of the center of diversity of the genus (southern Spain and northern Morocco) is Narcis- sus papyraceus Ker-Gawler. The species is self-incompatible and exhibits style dimorphism and a differential geographic distribution of style morph types (Arroyo et al., 2002) and peri- anth traits (Pérez-Barrales et al., 2007, 2009). Here we charac- terize 11 new polymorphic microsatellite loci for N. papyraceus and their cross-amplification in eight other Narcissus species collected in the field. These markers will be used to assess the genetic differentiation and structure across the species’ distri- bution range, and conduct paternity analyses to investigate the ecological and genetic mechanisms underlying variation in style polymorphism.