English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/51626
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Controlled autolysis facilitates the polyhydroxyalkanoate recovery in Pseudomonas putida KT2440

AuthorsMartínez, Virginia ; García, Pedro ; García, José Luis ; Prieto, María Auxiliadora
Issue DateJul-2011
PublisherBlackwell Publishing
CitationMicrobial Biotechnology 4(4): 533-547(2011)
AbstractThe development of efficient recovery processes is essential to reduce the cost of polyhydroxyalkanoates (PHAs) production. In this work, a programmed self-disruptive Pseudomonas putida BXHL strain, derived from the prototype medium-chain-length PHA producer bacterium P. putida KT2440, was constructed as a proof of concept for exploring the possibility to control and facilitate the release of PHA granules to the extracellular medium. The new autolytic cell disruption system is based on two simultaneous strategies: the coordinated action of two proteins from the pneumococcal bacteriophage EJ-1, an endolysin (Ejl) and a holin (Ejh), and the mutation of the tolB gene, which exhibits alterations in outer membrane integrity that induce lysis hypersensitivity. The ejl and ejh coding genes were expressed under a XylS/Pm monocopy expression system inserted into the chromosome of the tolB mutant strain, in the presence of 3-methylbenzoate as inducer molecule. Our results demonstrate that the intracellular presence of PHA granules confers resistance to cell envelope. Conditions to control the cell autolysis in P. putida BXHL in terms of optimal fermentation, PHA content and PHA recovery have been set up by exploring the sensitivity to detergents, chelating agents and wet biomass solubility in organic solvents such as ethyl acetate
Description15 páginas, 10 figuras, 3 tablas -- PAGS nros. 533-547
Publisher version (URL)http://dx.doi.org/0.1111/j.1751-7915.2011.00257.x
URIhttp://hdl.handle.net/10261/51626
DOI10.1111/j.1751-7915.2011.00257.x
ISSN1751-7907
E-ISSN1751-7915
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
Mart-nez_et_al-2011-Microbial_Biotechnology.pdf1,23 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.