English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/5161
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 78 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Eng1p, an Endo-1,3-β-Glucanase Localized at the Daughter Side of the Septum, Is Involved in Cell Separation in Saccharomyces cerevisiae
Autor : Baladrón, Victoriano; Ufano, Sandra; Dueñas, Encarnación; Martín-Cuadrado, Ana Belén; Rey Iglesias, Francisco del; Vázquez de Aldana, Carlos R.
Fecha de publicación : oct-2002
Editor: American Society for Microbiology
Citación : Eukaryot Cell 1(5): 774–786 (2002)
Resumen: ENG1 (YNR067c), a gene encoding a new endo-1,3-β-glucanase, was cloned by screening a genomic library with a DNA probe obtained by PCR with synthetic oligonucleotides designed according to conserved regions found between yeast exo-1,3-β-glucanases (Exg1p, Exg2p, and Ssg1p). Eng1p shows strong sequence similarity to the product of the Saccharomyces cerevisiae ACF2 gene, involved in actin assembly “in vitro,” and to proteins present in other yeast and fungal species. It is also related to plant glucan-binding elicitor proteins, which trigger the onset of a defense response upon fungal infection. Eng1p and Acf2p/Eng2p are glucan-hydrolyzing proteins that specifically act on 1,3-β linkages, with an endolytic mode of action. Eng1p is an extracellular, heavily glycosylated protein, while Acf2p/Eng2p is an intracellular protein with no carbohydrate linked by N-glycosidic bonds. ENG1 transcription fluctuates periodically during the cell cycle; maximal accumulation occurs during the M/G1 transition and is dependent on the transcription factor Ace2p. Interestingly, eng1 deletion mutants show defects in cell separation, and Eng1p localizes asymmetrically to the daughter side of the septum, suggesting that this protein is involved, together with chitinase, in the dissolution of the mother-daughter septum.
Versión del editor: http://dx.doi.org/10.1128/EC.1.5.774-786.2002
URI : http://hdl.handle.net/10261/5161
DOI: 10.1128/EC.1.5.774-786.2002
ISSN: 1535-9778
E-ISSN: 1535-9786
Aparece en las colecciones: (IMB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.