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Pediocin production by Pediococcus acidilactici in solid state culture on a waste medium: Process simulation and experimental results

AutorVázquez, José Antonio ; González Fernández, Pilar ; Murado García, Miguel Anxo
Palabras clavePediocin production
Solid-state fermentation
Waste medium
Fecha de publicación2004
EditorJohn Wiley & Sons
CitaciónBiotechnology and Bioengineering 85(6): 676-682 (2004)
ResumenThe production of pediocin by Pediococcus acidilactici was comparatively studied in submerged and solid-state culture, using polyurethane foam particles soaked in commercial (MRS) and waste media with various supplements, where product concentrations were 15 times higher in MRS medium. For the solid state analysis, cultures were treated by successive compression and refilling of tubular minireactors equipped with a piston, without the need for reinoculation. This method was found to be simple, reproducible, and easily controllable, allowing culture productivity to be maintained for long periods of time without alterations in the basic properties of the system. In addition, yields were found to be superior compared to those from submerged culture. The system kinetics were modeled on the basis of widely accepted assumptions with a good fit to the experimental results and observed biomass fluctuations less evident than those predicted by the kinetic model
Descripción7 páginas, 6 figuras
Versión del editorhttp://dx.doi.org/10.1002/bit.20018
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