Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/5154
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Rho4 GTPase is involved in secretion of glucanases during fission yeast cytokinesis

AuthorsSantos Romero, Beatriz CSIC ORCID; Martín-Cuadrado, Ana Belén; Vázquez de Aldana, Carlos R. CSIC ORCID; Rey Iglesias, Francisco del; Pérez González, Pilar
Issue DateOct-2005
PublisherAmerican Society for Microbiology
CitationEukaryot Cell 4(10): 1639–1645 (2005)
AbstractRho GTPases are regulators of signaling pathways that control actin organization and cell polarity processes in all eukaryotic cells. In Schizosaccharomyces pombe, Rho4p is involved in the regulation of septum degradation during cytokinesis. Here we show that Rho4p participates in the secretion of the glucanases Eng1p and Agn1p, which are responsible for the septum degradation. First, eng1+ or agn1+ overexpression suppressed the rho4Δ multiseptation phenotype, and simultaneous overproduction of Rho4p and Eng1p or of Rho4p and Agn1p caused a dramatic lysis. Second, Rho4p was not necessary for Eng1p-mediated glucanase activity as measured in cell extracts; however, rho4Δ cells have a lower level of (1,3)-β-d-glucanase activity in the culture medium. Additionally, Eng1- or Agn1-green fluorescent protein did not properly localize to the septum in rho4Δ cells grown at 37°C. There was a decreased amount of these enzymes in the cell wall and in the culture medium of rho4Δ cells at 37°C. These results provide evidence that Rho4p is involved in the regulation of Eng1p and Agn1p secretion during cytokinesis.
Publisher version (URL)http://dx.doi.org/10.1128/EC.4.10.1639-1645.2005
URIhttp://hdl.handle.net/10261/5154
DOI10.1128/EC.4.10.1639-1645.2005
ISSN1535-9778
Appears in Collections:(IMB) Artículos

Files in This Item:
File Description SizeFormat
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

PubMed Central
Citations

17
checked on May 14, 2022

SCOPUSTM   
Citations

33
checked on May 15, 2022

WEB OF SCIENCETM
Citations

32
checked on May 11, 2022

Page view(s)

344
checked on May 18, 2022

Download(s)

102
checked on May 18, 2022

Google ScholarTM

Check

Altmetric

Dimensions


Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.