English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/51466
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Título

Assessment of microbial populations dynamics in a blue cheese by culturing and denaturing gradient gel electrophoresis

AutorAlegría, Ángel ; González, Renata; Díaz Fernández, José Mario; Mayo Pérez, Baltasar
Palabras claveFood microbiology
Biotechnology
Photobiology
Cheese microbiology
DGGE
Blue-veined cheese
Fecha de publicaciónmar-2011
EditorSpringer
CitaciónCurrent Microbiology 62(3): 888-893 (2011)
ResumenThe composition and development of microbial population during the manufacture and ripening of two batches of a blue-veined cheese was examined by culturing and polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) (PCR-DGGE). Nine selective and/or differential media were used to track the cultivable populations of total and indicator microbial groups. For PCR-DGGE, the V3 hyper variable region of the bacterial 16S rRNA gene and the eukaryotic D1 domain of 28S rDNA were amplified with universal primers, specific for prokaryotes and eukaryotes, respectively. Similarities and differences between the results obtained by the culturing and the molecular method were recorded for some populations. Culturing analysis allows minority microbial groups (coliforms, staphylococci) to be monitored, although in this study PCR-DGGE identified a population of Streptococcus thermophilus that went undetected by culturing. These results show that the characterization of the microbial populations interacting and evolving during the cheese-making process is improved by combining culturing and molecular methods. © 2010 Springer Science+Business Media, LLC.
Versión del editorhttp://dx.doi.org/10.1007/s00284-010-9799-7
URIhttp://hdl.handle.net/10261/51466
DOI10.1007/s00284-010-9799-7
Identificadoresdoi: 10.1007/s00284-010-9799-7
issn: 0343-8651
e-issn: 1432-0991
Aparece en las colecciones: (IPLA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
assessment_microbial_Alegria.pdf319,28 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.