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Título

Enhancement of nisin production in milk by conjugal transfer of the protease-lactose plasmid pLP712 to the wild strain Lactococcus lactis UQ2

AutorGarcía-Parra, María D.; Campelo, Ana B. ; García-Almendárez, Blanca E.; Regalado, Carlos; Rodríguez González, Ana ; Martínez Fernández, Beatriz
Palabras claveDairy starter
Nisin
Lactococcus lactis
pLP712
Conjugation
Fecha de publicaciónnov-2010
EditorSociety of Dairy Technology
CitaciónInternational Journal of Dairy Technology 63(4): 523-529 (2010)
ResumenLactococcus lactis UQ2 is a wild nisin A producer isolated from a Mexican cheese that grows poorly in milk. Conjugal matings with L. lactis NCDO712 to transfer the Lac+ Prt+ plasmid pLP712 and selection with nisin and lactose yielded L. lactis NCDO712 NisA+. Naturally rifampicin resistant L. lactis UQ2Rif was isolated to provide an additional selective marker. The identity of a transconjugant L. lactis UQ2Rif Lac+ was confirmed by RAPD-PCR fingerprinting, nisA PCR amplification, nisin production, presence of pLP712 and phospho-β-galactosidase activity. This strain performed well in milk and synthesised 200 IU/mL nisin, 40 times more than the original strain. © 2010 Society of Dairy Technology.
URIhttp://hdl.handle.net/10261/51409
DOI10.1111/j.1471-0307.2010.00618.x
Identificadoresdoi: 10.1111/j.1471-0307.2010.00618.x
issn: 1364-727X
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