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Analytical method for quantification β-cyclodextrin in milk, cream and butter by LC

AutorAlonso López, Leocadio ; Cuesta Alonso, Emilia Paloma; Gilliland, S. E.
Palabras claveColumn liquid chromatography
Dairy products
β-Cyclodextrin
Fecha de publicación2009
EditorVieweg Verlag
CitaciónChromatographia 69(9-10): 1089-1092 (2009)
ResumenA simple, rapid and sensitive liquid chromatographic method using a refractive index detector has been developed and validated for identification and quantification of β-cyclodextrin in milk, cream and butter. The chromatographic system consists of a YMC ODS-AQ column packed with C18 reversed phase silica packing material as stationary phase using a mixture of methanol and water 7:93% (v/v) as the mobile phase. Linearity was established for the β-cyclodextrin concentration in the range 0.01-4 mg mL-1, with a coefficient (r) of 0.9998. Repeatability of the method was assessed; the coefficient of variation for β-cyclodextrin range were 1.24, 3.01, and 5.36% for milk, cream and butter. Recoveries ranged between 99.91 and 94.53%. This method was highly reproducible and reliable for quantification of treated milk with β-cyclodextrin for removal cholesterol from the milk fat. © 2009 Vieweg+Teubner | GWV Fachverlage GmbH.
URIhttp://hdl.handle.net/10261/51346
DOI10.1365/s10337-009-1022-2
Identificadoresdoi: 10.1365/s10337-009-1022-2
issn: 0009-5893
e-issn: 1612-1112
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