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dc.contributor.authorGriffith, Janice-
dc.contributor.authorPeñalva, Miguel Ángel-
dc.contributor.authorReggiori, Fulvio-
dc.identifier.citationJournal of Electron Microscopy 60(3):211-216(2011)es_ES
dc.description6 páginas, 2 figuras -- PAGS nros. 211-216es_ES
dc.description.abstractThe study of filamentous fungi is fundamental not only to extend their biotechnological applications, but also to develop new drugs to fight pathological species. Morphological analyses are particularly relevant when investigating their development and differentiation. The need to maintain the orientation of hyphae and the presence of a cell wall, which hampers the sample infiltration with cryoprotectants and other reagents necessary to preserve the cell ultrastructure, creates difficulties with the use of electron microscopy (EM). Here, we present an immunoelectron microscopy (IEM) procedure that combines the Tokuyasu protocol adapted to yeast and the flat-embedding technique. While the first method leads to a fine resolution of the ultrastructure of Aspergillus nidulans because of both the cell wall permeabilization and the negative membrane coloration, the second permits us to preserve the spatial distribution of the hypahe of this fungus. The presented data demonstrate the advantages of this combination and the unprecedented potential of this relatively simple and rapid protocol in resolving the morphology of filamentous fungi and performing localization studiees_ES
dc.description.sponsorshipM.A.P. was supported by the MCIN BIO2009-07281 and the Comunidad de Madrid SAL/0246/2006 grants. F.R. was supported by the Netherlands Organization for Health Research and Development (ZonMW-VIDI-917.76.329), by the Netherlands Organization for Scientific Research (Chemical Sciences, ECHO grant-700.59.003) and by the Utrecht University (high potential grant)es_ES
dc.publisherOxford University Presses_ES
dc.subjectfilamentous fungies_ES
dc.subjectAspergillus nidulanses_ES
dc.subjectimmunogold labellinges_ES
dc.subjectTokuyasu methodes_ES
dc.titleAdaptation of the Tokuyasu method for the ultrastructural study and immunogold labelling of filamentous fungies_ES
dc.description.peerreviewedPeer reviewedes_ES
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