Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/51345
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Griffith, Janice | - |
dc.contributor.author | Peñalva, Miguel Ángel | - |
dc.contributor.author | Reggiori, Fulvio | - |
dc.date.issued | 2011-06 | - |
dc.identifier.citation | Journal of Electron Microscopy 60(3):211-216(2011) | es_ES |
dc.identifier.issn | 0022-0744 | - |
dc.identifier.uri | http://hdl.handle.net/10261/51345 | - |
dc.description | 6 páginas, 2 figuras -- PAGS nros. 211-216 | es_ES |
dc.description.abstract | The study of filamentous fungi is fundamental not only to extend their biotechnological applications, but also to develop new drugs to fight pathological species. Morphological analyses are particularly relevant when investigating their development and differentiation. The need to maintain the orientation of hyphae and the presence of a cell wall, which hampers the sample infiltration with cryoprotectants and other reagents necessary to preserve the cell ultrastructure, creates difficulties with the use of electron microscopy (EM). Here, we present an immunoelectron microscopy (IEM) procedure that combines the Tokuyasu protocol adapted to yeast and the flat-embedding technique. While the first method leads to a fine resolution of the ultrastructure of Aspergillus nidulans because of both the cell wall permeabilization and the negative membrane coloration, the second permits us to preserve the spatial distribution of the hypahe of this fungus. The presented data demonstrate the advantages of this combination and the unprecedented potential of this relatively simple and rapid protocol in resolving the morphology of filamentous fungi and performing localization studie | es_ES |
dc.description.sponsorship | M.A.P. was supported by the MCIN BIO2009-07281 and the Comunidad de Madrid SAL/0246/2006 grants. F.R. was supported by the Netherlands Organization for Health Research and Development (ZonMW-VIDI-917.76.329), by the Netherlands Organization for Scientific Research (Chemical Sciences, ECHO grant-700.59.003) and by the Utrecht University (high potential grant) | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | filamentous fungi | es_ES |
dc.subject | hyphae | es_ES |
dc.subject | Aspergillus nidulans | es_ES |
dc.subject | Cryosectioning | es_ES |
dc.subject | Immunogold labelling | es_ES |
dc.subject | Tokuyasu method | es_ES |
dc.title | Adaptation of the Tokuyasu method for the ultrastructural study and immunogold labelling of filamentous fungi | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1093/jmicro/dfr026 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1093/jmicro/dfr026 | es_ES |
dc.identifier.e-issn | 1477-9986 | - |
dc.embargo.terms | 2012-06-01 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
Aparece en las colecciones: | (CIB) Artículos |
CORE Recommender
SCOPUSTM
Citations
9
checked on 24-mar-2024
WEB OF SCIENCETM
Citations
8
checked on 25-feb-2024
Page view(s)
358
checked on 29-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.