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dc.contributor.author | Valdearcos, Martín | - |
dc.contributor.author | Esquinas, Esperanza | - |
dc.contributor.author | Meana, Clara | - |
dc.contributor.author | Gil-de-Gómez, Luis | - |
dc.contributor.author | Guijas, Carlos | - |
dc.contributor.author | Balsinde, Jesús | - |
dc.contributor.author | Balboa, María A. | - |
dc.date.accessioned | 2012-06-12T09:13:20Z | - |
dc.date.available | 2012-06-12T09:13:20Z | - |
dc.date.issued | 2011 | - |
dc.identifier | doi: 10.4049/¿jimmunol.1003279 | - |
dc.identifier | issn: 0022-1767 | - |
dc.identifier | e-issn: 1550-6606 | - |
dc.identifier.citation | Journal of Immunology 186(10): 6004-6013 (2011) | - |
dc.identifier.uri | http://hdl.handle.net/10261/51340 | - |
dc.description.abstract | The lipins have been described as metabolic enzymes that regulate lipid biosynthesis and also signaling processes by controlling the cellular concentration of bioactive lipids, phosphatidic acid, and diacylgycerol. In the present work we have studied the subcellular localization and role of lipin-1 in human monocyte-derived macrophages. Human macrophages express lipin-1 isoforms α and β. A transfected lipin-1α–enhanced GFP construct associates with membranes of cellular organelles that can be stained with Nile Red. Colocalization experiments with lipid droplet (LD)-specific proteins such as adipophilin/adipose differentiation-related protein/perilipin 2 or TIP47/perilipin 3 show that both proteins colocalize with lipin-1α in the same cellular structures. Reduction of the expression levels of lipin-1 by small interfering RNA technology does not impair triacylglycerol biosynthesis but reduces the size of LDs formed in response to oleic acid. In agreement with these data, peritoneal macrophages from animals that carry a mutation in the Lpin-1 gene (fld animals) also produce less and smaller LDs in response to oleic acid. Mass spectrometry determinations demonstrate that the fatty acid composition of triacylglycerol in isolated LDs from lipin-1–deficient cells differs from that of control cells. Moreover, activation of cytosolic group IVA phospholipase A2α, a proinflammatory enzyme that is also involved in LD biogenesis, is also compromised in lipin-1–deficient cells. Collectively, these data suggest that lipin-1 associates with LDs and regulates the activation of cytosolic group IVA phospholipase A2α in human monocyte-derived macrophages. | - |
dc.description.sponsorship | This work was supported by the Spanish Ministry of Science and Innovation (Grants SAF2007-60055, BFU2007-67154, SAF2010-18831, and BFU2010-18826). M.V. was supported by a predoctoral fellowship from the Regional Government of Castile and Leon. E.E. was supported by a predoctoral fellowship from the Spanish National Research Council (Junta de Ampliación de Estudios Program). L.G.-d.-G. was supported by a predoctoral fellowship from the Spanish Ministry of Science and Innovation (Formación de Personal Investigador Program). | - |
dc.language.iso | eng | - |
dc.publisher | American Association of Immunologists | - |
dc.relation.isversionof | Preprint | - |
dc.rights | openAccess | - |
dc.title | Subcellular Localization and Role of Lipin-1 in Human Macrophages | - |
dc.type | artículo | - |
dc.identifier.doi | 10.4049/¿jimmunol.1003279 | - |
dc.relation.publisherversion | http://dx.doi.org/10.4049/¿jimmunol.1003279 | - |
dc.date.updated | 2012-06-12T09:13:20Z | - |
dc.description.version | Peer Reviewed | - |
dc.contributor.funder | Junta de Castilla y León | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100014180 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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Subcellular Localization.pdf | 5,16 MB | Adobe PDF | Visualizar/Abrir |
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