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Detection of the early stage of recombinational DNA repair by silicon nanowire transistors

AutorChiesa, Marco ; Cardenas, P. P.; Otón, Francisco ; Martínez, Javier ; Mas Torrent, Marta ; García-Pérez, Fernando ; Alonso, Juan Carlos ; Rovira, Concepció ; García García, Ricardo
Fecha de publicación2012
EditorAmerican Chemical Society
CitaciónNano Letters 12(3): 1275-1281 (2012)
ResumenA silicon nanowire-based biosensor has been designed and applied for label-free and ultrasensitive detection of the early stage of recombinational DNA repair by RecA protein. Silicon nanowires transistors were fabricated by atomic force microscopy nanolithography and integrated into a microfluidic environment. The sensor operates by measuring the changes in the resistance of the nanowire as the biomolecular reactions proceed. We show that the nanoelectronic sensor can detect and differentiate several steps in the binding of RecA to a single-stranded DNA filament taking place on the nanowire-aqueous interface. We report relative changes in the resistance of 3.5% which are related to the interaction of 250 RecA·single-stranded DNA complexes. Spectroscopy data confirm the presence of the protein-DNA complexes on the functionalized silicon surfaces. © 2012 American Chemical Society.
Versión del editorhttp://dx.doi.org/10.1021/nl2037547
URIhttp://hdl.handle.net/10261/51337
DOI10.1021/nl2037547
Identificadoresissn: 1530-6984
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