Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/51329
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dc.contributor.authorRevuelta, Luis-
dc.contributor.authorOrtego, Félix-
dc.contributor.authorDíaz-Ruíz, José Ramón-
dc.contributor.authorCastañera, Pedro-
dc.contributor.authorTenllado, Francisco-
dc.contributor.authorHernández-Crespo, Pedro-
dc.date.accessioned2012-06-12T08:17:56Z-
dc.date.available2012-06-12T08:17:56Z-
dc.date.issued2011-10-
dc.identifier.citationInsect Biochemistry and Molecular Biology 41(10):795-803(2011)es_ES
dc.identifier.issn0965-1748-
dc.identifier.urihttp://hdl.handle.net/10261/51329-
dc.description26 p.-6 fig.es_ES
dc.description.abstractThe Colorado potato beetle (CPB), Leptinotarsa decemlineata is an important economic pest of potato worldwide. Resistance to organophosphates and carbamates in CPB has been associated in some cases to point mutations in the acetylcholinesterase (AChE) gene Ldace2, an orthologue of Drosophila melanogaster Dmace2. In this paper we report cloning and sequencing of Ldace1, an orthologue of Anopheles gambiae Agace1 that was previously unknown in CPB. The Ldace1 coding enzyme contains all residues conserved in a functionally active AChE. Ldace1 is expressed at higher levels (between 2- and 11-fold) than Ldace2 in embryos, in the four larval instars and in adults. Specific interference of Ldace1 by means of dsRNA injection resulted in a reduction of AChE activity to an approximate 50% compared to control, whilst interference of Ldace2 reduced AChE activity to an approximate 85%. Analysis of zymograms of AChE activity after interference indicates that LdAChE1 is the enzyme predominantly responsible for the activity visualised. Interference of Ldace1 in CPB adults caused a significant increase in mortality (43%) as early as three days post-injection (p.i.), suggesting the essential role of Ldace1. Interference of Ldace2 also caused a significant increase in mortality (29%) compared to control, although at seven days p.i. The effect of the interference of Ldace1 on susceptibility to the organophosphate chlorpyrifos points out that LdAChE1 could be a main target for this insecticide. In the light of our results, studies associating resistance in CPB to mutations in Ldace2 should be reviewed, taking into consideration analysis of the Ldace1 genees_ES
dc.description.sponsorshipThis work has been supported by MICINN Grants BIO2003-03428 and BIO2009-10172es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsopenAccesses_ES
dc.subjectLeptinotarsa decemlineataes_ES
dc.subjectInsecticide targetes_ES
dc.subjectAcetylcholinesterasees_ES
dc.subjectRNA interferencees_ES
dc.subjectDouble-stranded RNAes_ES
dc.subjectGene silencinges_ES
dc.titleContribution of Ldace1 gene to acetylcholinesterase activity in Colorado potato beetlees_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.ibmb.2011.06.001-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.ibmb.2011.06.001es_ES
dc.identifier.e-issn1879-0240-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.openairetypeartículo-
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