English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/5124
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 11 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Transcriptomal profiling of site-specific Ras signals
Autor : Agudo-Ibáñez, Lorena; Núñez, Fátima; Calvo, Fernando; Berenjeno, Inmaculada M.; Bustelo, Xosé R.; Crespo, Piero
Palabras clave : Ras
Compartmentalization
Gene microarrays
Transformation
Fecha de publicación : nov-2007
Editor: Elsevier
Citación : Cellular Signalling 19(11): 2264–2276 (2007)
Resumen: Ras proteins are distributed in distinct plasma-membrane microdomains and endomembranes. The biochemical signals generated by Ras therein differ qualitatively and quantitatively, but the extent to which this spatial variability impacts on the genetic program switched-on by Ras is unknown. We have used microarray technology to identify the transcriptional targets of localization-specific Ras subsignals in NIH3T3 cells expressing H-RasV12 selectively tethered to distinct cellular microenvironments. We report that the transcriptomes resulting from site-specific Ras activation show a significant overlap. However, distinct genetic signatures can also be found for each of the Ras subsignals. Our analyses unveil 121 genes uniquely regulated by Ras signals emanating from plasma-membrane microdomains. Interestingly, not a single gene is specifically controlled by lipid raft-anchored Ras. Furthermore, only 9 genes are exclusive for Ras signals from endomembranes. Also, we have identified 31 genes common to the site-specific Ras subsignals capable of inducing cellular transformation. Among these are the genes coding for Vitamin D receptor and for p120-GAP and we have assessed their impact in Ras-induced transformation. Overall, this report reveals the complexity and variability of the different genetic programs orchestrated by Ras from its main sublocalizations.
Descripción : El pdf del artículo es el manuscrito de autor.
Versión del editor: http://dx.doi.org/10.1016/j.cellsig.2007.06.025
URI : http://hdl.handle.net/10261/5124
DOI: 10.1016/j.cellsig.2007.06.025
ISSN: 0898-6568
Aparece en las colecciones: (IBBTEC) Artículos
(IBMCC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
ras.pdf2,14 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.