English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/51205
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Identification of Neotropical felid faeces using RCP-PCR

AutorRoques, Séverine ; Chávez, Cuauhtémoc; Keller, Claudia; Magnusson, W.E.; Palomares, Francisco ; Godoy, José A.
Palabras claveFelidae
noninvasive
species identification
mitochondrial
Fecha de publicación2011
EditorJohn Wiley & Sons
ResumenFaeces similarity among sympatric felid species has generally hampered their use in distributional, demographic and die- tary studies. Here, we present a new and simple approach based on a set of species-specific primers, for the unambiguous identification of faeces from sympatric neotropical felids (i.e. puma, jaguar, jaguarundi and ocelot ⁄ margay). This method, referred to as rapid classificatory protocol-PCR (RCP-PCR), consists of a single-tube multiplex PCR yielding species-spe- cific banding patterns on agarose gel. The method was optimized with samples of known origin (14 blood and 15 fresh fae- ces) and validated in faecal samples of unknown origin (n = 138), for some of which (n = 40) we also obtained species identification based on mtDNA sequencing. This approach proved reliable and provides high identification success rates from faeces. Its simplicity and cost effectiveness should facilitate its application for routine surveys of presence and abun- dance of these species.
Versión del editorhttp://dx.doi.org/10.1111/j.1755-0998.2010.02878.x
URIhttp://hdl.handle.net/10261/51205
DOI10.1111/j.1755-0998.2010.02878.x
Aparece en las colecciones: (EBD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
roques et al_mol ecol_2011.doc594,5 kBMicrosoft WordVisualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.