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Título

Genetic and molecular analysis of mitotic recombination in Saccharomyces cerevisiae

AutorGómez-González, Belén ; Ruiz, José F. ; Aguilera, Andrés
Fecha de publicación2011
EditorSpringer
CitaciónDNA Recombination: 151-172 (2011)
SerieMethods in Mmolecular Biology 745
ResumenMany systems have been developed for the study of mitotic homologous recombination (HR) in the yeast Saccharomyces cerevisiae at both genetic and molecular levels. Such systems are of great use for the analysis of different features of HR as well as of the effect of mutations, transcription, etc., on HR. Here we describe a selection of plasmid- and chromosome-borne DNA repeat assays, as well as plasmid-chromosome recombination systems, which are useful for the analysis of spontaneous and DSB-induced recombination. They can easily be used in diploid and, most importantly, in haploid yeast cells, which is a great advantage to analyze the effect of recessive mutations on HR. Such systems were designed for the analysis of a number of different HR features, which include the frequency and length of the gene conversion events, the frequency of reciprocal exchanges, the proportion of gene conversion versus reciprocal exchange, or the molecular analysis of sister chromatid exchange.
URIhttp://hdl.handle.net/10261/51087
DOI10.1007/978-1-61779-129-1_10
Identificadoresisbn: 978-1-61779-129-1
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