English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/50788
Título

Klebsiella pneumoniae subverts the activation of inflammatory responses in a NOD1-dependent manner

AutorRegueiro, Verónica; Moranta, David; Frank, Christian G.; Larrarte, Eider; Margareto, Javier; March, Catalina; Garmendia, Juncal ; Bengoechea, José Antonio
Fecha de publicación2011
EditorBlackwell Publishing
CitaciónCellular Microbiology 13(1): 135-153 (2011)
ResumenKlebsiella pneumoniae is an important cause of community-acquired and nosocomial pneumonia. Subversion of inflammation is essential for pathogen survival during infection. Evidence indicates that K. pneumoniae infections are characterized by lacking an early inflammatory response although the molecular bases are currently unknown. Here we unveil a novel strategy employed by a pathogen to counteract the activation of inflammatory responses. K. pneumoniae attenuates pro-inflammatory mediators-induced IL-8 secretion. Klebsiella antagonizes the activation of NF-κB via the deubiquitinase CYLD and blocks the phosphorylation of mitogen-activated protein kinases (MAPKs) via the MAPK phosphatase MKP-1. Our studies demonstrate that K. pneumoniae has evolved the capacity to manipulate host systems dedicated to control the immune balance. To exert this anti-inflammatory effect, Klebsiella engages NOD1. In NOD1 knock-down cells, Klebsiella neither induces the expression of CYLD and MKP-1 nor blocks the activation of NF-κB and MAPKs. Klebsiella inhibits Rac1 activation; and inhibition of Rac1 activity triggers a NOD1-mediated CYLD and MKP-1 expression which in turn attenuates IL-1β-induced IL-8 secretion. A capsule (CPS) mutant does not attenuate the inflammatory response. However, purified CPS neither reduces IL-1β-induced IL-8 secretion nor induces the expression of CYLD and MKP-1 thereby indicating that CPS is necessary but not sufficient to attenuate inflammation. © 2010 Blackwell Publishing Ltd.
URIhttp://hdl.handle.net/10261/50788
DOI10.1111/j.1462-5822.2010.01526.x
Identificadoresdoi: 10.1111/j.1462-5822.2010.01526.x
issn: 1462-5814
Aparece en las colecciones: (IDAB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.