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Proteomic analysis of 1α,25-Dihydroxyvitamin D3 action on human colon cancer cells reveals a link to splicing regulation

AuthorsCristobo, Iván; Larriba, María Jesús ; Ríos, Vivian de los ; García, Francisco; Muñoz Terol, Alberto ; Casal, J. Ignacio
KeywordsVitamin D
Colon cancer
Nuclear proteins
Issue Date21-Dec-2011
European Proteomics Association
CitationJournal of Proteomics 75(2): 384-397 (2011)
Abstract1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3) and other vitamin D compounds are promising molecules in the prevention and therapy of colon cancer and other neoplasias. To study the mechanism of action of 1,25(OH)2D3 in colon cancer cells, we carried out a comparative proteomic analysis of the nuclear fractions of SW480-ADH cells treated with 1,25(OH)2D3 or vehicle during 8 or 48 h. 2D-DIGE analysis combined with MALDI-TOF-TOF mass spectrometry interrogation led to the identification of 59 differentially expressed unique proteins. Most identified proteins were nuclear, but several cytoskeleton-associated proteins were also detected. A good concordance between changes in expression at protein and RNA levels was observed for the validated proteins. A large group of identified proteins, such as SFPQ, SMARCE, KHSRP, TARDBP and PARP1, were involved in RNA processing or modification and have been ascribed to the spliceosome compartment of human cells. In addition, a smaller group of proteins (ERM (Ezrin, Radixin, Moesin) family, VCL, CORO1C, ACTB) were cytoskeleton-associated and played a role in cell adhesion and morphology. These results confirm the induction of epithelial phenotype by 1,25(OH)2D3 and suggest a role for vitamin D compounds in the regulation of the spliceosome and thus, in alternative splicing and possibly microRNA synthesis in colon cancer cells.
Graphical abstract Proteomic analysis of 1α,25-Dihydroxyvitamin D3 action on human colon cancer cells reveals a link to splicing regulation. View high quality image (147K)
Description14 páginas, 6 figuras, 2 tablas.
El pdf del artículo es la versión pre-print.
Publisher version (URL)http://dx.doi.org/10.1016/j.jprot.2011.08.003
Appears in Collections:(CIB) Artículos
(IIBM) Artículos
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