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Title

Molecular tools for species discrimination and detection of hybridization between two closely related Clupeid fishes Alosa alosa and A. fallax

AuthorsFaria, Rui; Pinheiro, Aluisio; Gabaldón, Toni; Weiss, Steven; Alexandrino, Paulo
Issue DateDec-2011
PublisherBlackwell Publishing
CitationJournal of Applied Ichthyology 27(s3): 16-20 (2011)
AbstractRevealing the distinction between natural and human-mediated hybridization is critical for implementing adequate conservation strategies. To discriminate between two shad species, Alosa alosa and A. fallax, and to assess levels of hybridization between them, we developed two independent genetic markers (cytochrome b and a nuclear fragment with putative functional relevance) using single strand conformation polymorphism (SSCP). The genotyping of two contiguous discriminative nuclear SNPs was further confirmed using a Taqman RT PCR assay. Both methodologies are shown to be very informative for species discrimination and reveal the occurrence of varying levels of introgression between European shads. In the Lima River, Portugal, where three morphological groups were identified (the two parental species and putative hybrids), A. alosa typical mtDNA haplotypes were found in A. fallax but not vice versa. Taking into account the different patterns observed, rivers-specific management strategies should be considered in the future. © 2011 Blackwell Verlag, Berlin.
Publisher version (URL)http://dx.doi.org/10.1111/j.1439-0426.2011.01846.x
URIhttp://hdl.handle.net/10261/50590
DOI10.1111/j.1439-0426.2011.01846.x
E-ISSN1439-0426
Identifiersdoi: 10.1111/j.1439-0426.2011.01846.x
issn: 0175-8659
Appears in Collections:(IBE) Artículos
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