English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/5056
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 45 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Gene function analysis in environmental isolates: The nif regulon of the strict iron oxidizing bacterium Leptospirillum ferrooxidans
Autor : Parro-García, Víctor ; Moreno-Paz, Mercedes
Palabras clave : Shotgun DNA microarrays
Gene expression
Nitrogen fixation
Iron oxidizer
Fecha de publicación : jun-2003
Editor: National Academy of Sciences (U.S.)
Citación : Proc Natl Acad Sci U S A. 2003 June 24; 100(13): 7883–7888
Resumen: A random genomic library from an environmental isolate of the Gram-negative bacterium Leptospirillum ferrooxidans has been printed on a microarray. Gene expression analysis was carried out with total RNA extracted from L. ferrooxidans cultures in the presence or absence of ammonium as nitrogen source under aerobic conditions. Although practically nothing is known about the genome sequence of this bacterium, this approach allowed us the selection and sequencing of only those clones bearing genes that showed an altered expression pattern. By sequence comparison, we have identified most of the genes of nitrogen fixation regulon in L. ferrooxidans, like the nifHDKENX operon, encoding the structural components of Mo-Fe nitrogenase; nifSU-hesB-hscBA-fdx operon, for Fe-S cluster assembly; the amtB gene (ammonium transporter); modA (molybdenum ABC type transporter); some regulatory genes like ntrC, nifA (the specific activator of nif genes); or two glnB-like genes (encoding the PII regulatory protein). Our results show that shotgun DNA microarrays are very powerful tools to accomplish gene expression studies with environmental bacteria whose genome sequence is still unknown, avoiding the time and effort necessary for whole genome sequencing projects.
Descripción : Copyright © by National Academy of Sciences. La versión original está disponible en http://www.pnas.org/
URI : http://hdl.handle.net/10261/5056
DOI: 10.1073/pnas.1230487100
ISSN: 1091-6490
Aparece en las colecciones: (CAB) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.