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Production of eukaryotic antimicrobial peptides by bacteria A review.

AutorGaiser, Rogier A. ; Rivas, Luis ; López, Paloma
Palabras claveeukaryotic
antimicrobial peptide
expression system
Escherichia coli
lactic acid bacteria
Fecha de publicación2011
CitaciónScience against microbial pathogens: communicating current research and technological advances, 2 : 992-1002
ResumenThe alarming increase in incidence of multidrug-resistant, pathogenic bacteria, the paucity in the discovery of new antibiotic leads, and the resulting public health risk have recently heightened interest in naturally occurring antimicrobial peptides (AMPs) as potential novel therapeutics. [1, 2] Higher organisms are equipped with an innate immune system that allows them to fight invading pathogens. Antimicrobial peptides, also referred to as host-defense peptides (HDP) [3], are gene-encoded, small effector molecules of the innate immune system, which play a major role as the first chemical barrier against invading pathogens. Their characteristic features are broad-spectrum antimicrobial activity and constitutive expression or fast induction after pathogen challenge. [4] Usually ranging between 13-45 amino acid residues long, these peptides share a net positive charge and amphipathic 3D structures (displaying both hydrophilic and hydrophobic areas), despite the large variety of their primary sequences. [5, 6] Three major families of AMPs have been described in humans: cathelicidins, defensins, and histatins. Overall, more than 1,200 AMP sequences have been reported from animals and plants. [7, 8] The heterologous expression of eukaryotic antimicrobial peptides (EAPs) by microorganisms could be a costeffective tool for functional and clinical research. The use of bacteria as cell-factories is interesting due to the possibility of isolating and purifying secreted proteins. [9] The Gram-positive lactic acid bacteria (LAB) have a generallyregarded- as-safe (GRAS)-status, and are widely employed in human food production, with a long history of safe use in a variety of fermentation processes. [10] Several studies have been performed on the use of LAB as mucosal delivery vehicles of heterologous proteins, such as vaccine antigens or therapeutic agents. [11, 12, 13] Recent advances in inducible promoters and selection markers in food-grade organisms open up possibilities to select safe organisms as probiotics. [14] This review focuses on currently available systems for the heterologous expression of functionally active eukaryotic antimicrobial peptides by bacteria, with a focus on potential future applications in LAB
Descripción11 páginas, 2 tablas -- PAGS nros. 978-988
ISBN13: 978-84-939843-2-8
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