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dc.contributor.authorVillagrasa, P.-
dc.contributor.authorDiaz, V.M.-
dc.contributor.authorViñas-Castells, R.-
dc.contributor.authorPeiro, S.-
dc.contributor.authorValle-Perez, Beatriz del-
dc.contributor.authorDave, N.-
dc.contributor.authorRodriguez-Asiain, A.-
dc.contributor.authorCasal, J. Ignacio-
dc.contributor.authorLizcano, J. M.-
dc.contributor.authorDuñach, Mireia-
dc.contributor.authorGarcía de Herreros, Antonio-
dc.identifier.citationOncogene 31:4022-4033 (2012)es_ES
dc.description12 p.-7 fig.es_ES
dc.description.abstractSnail1 is a transcriptional factor essential for triggering epithelial-to-mesenchymal transition. Moreover, Snail1 promotes resistance to apoptosis, an effect associated to PTEN gene repression and Akt stimulation. In this article we demonstrate that Snail1 activates Akt at an additional level, as it directly binds to and activates this protein kinase. The interaction is observed in the nucleus and increases the intrinsic Akt activity. We determined that Akt2 is the isoform interacting with Snail1, an association that requires the pleckstrin homology domain in Akt2 and the C-terminal half in Snail1. Snail1 enhances the binding of Akt2 to the E-cadherin (CDH1) promoter and Akt2 interference prevents Snail1 repression of CDH1 gene. We also show that Snail1 binding increases Akt2 intrinsic activity on histone H3 and have identified Thr45 as a residue modified on this protein. Phosphorylation of Thr45 in histone H3 is sensitive to Snail1 and Akt2 cellular levels; moreover, Snail1 upregulates the binding of phosphoThr45 histone H3 to the CDH1 promoter. These results uncover an unexpected role of Akt2 in transcriptional control and point out to phosphorylation of Thr45 in histone H3 as a new epigenetic mark related to Snail1 and Akt2 action.Oncogene advance online publicationes_ES
dc.description.sponsorshipThis study was supported by grants awarded by the Ministerio de Ciencia e Innovación (BFU2006-03203 and BFU2009-07578 to MD and SAF2006-00339 and SAF2010-16089 to AGH) and Fundació La Marató de TV3 (to AGH). The partial support from the Instituto Carlos III-Fondos FEDER (RTICCC, C03710, RD06/0020/0040) and the Generalitat de Catalunya (2009SGR867) is also appreciated. PV and RV-C were supported by predoctoral fellowships from the Ministerio de Ciencia y Tecnología and Instituto Carlos III, respectivelyes_ES
dc.publisherNature Publishing Groupes_ES
dc.titleAKT2 interacts with Snail1 in the E-cadherin Promoteres_ES
dc.relation.publisherversionhttp://dx.doi.org/ 10.1038/onc.2011.562es_ES
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