English
español
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10261/50517
Share/Impact:
Statistics |
![]() ![]() ![]() |
|
|
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |||
|
Title: | AKT2 interacts with Snail1 in the E-cadherin Promoter |
Authors: | Villagrasa, P.; Diaz, V.M.; Viñas-Castells, R.; Peiro, S.; Valle-Perez, Beatriz del; Dave, N.; Rodriguez-Asiain, A.; Casal, J. Ignacio ![]() |
Keywords: | Snail1 EMT Akt E-cadherin |
Issue Date: | 12-Dec-2011 |
Publisher: | Nature Publishing Group |
Citation: | Oncogene 31:4022-4033 (2012) |
Abstract: | Snail1 is a transcriptional factor essential for triggering epithelial-to-mesenchymal transition. Moreover, Snail1 promotes resistance to apoptosis, an effect associated to PTEN gene repression and Akt stimulation. In this article we demonstrate that Snail1 activates Akt at an additional level, as it directly binds to and activates this protein kinase. The interaction is observed in the nucleus and increases the intrinsic Akt activity. We determined that Akt2 is the isoform interacting with Snail1, an association that requires the pleckstrin homology domain in Akt2 and the C-terminal half in Snail1. Snail1 enhances the binding of Akt2 to the E-cadherin (CDH1) promoter and Akt2 interference prevents Snail1 repression of CDH1 gene. We also show that Snail1 binding increases Akt2 intrinsic activity on histone H3 and have identified Thr45 as a residue modified on this protein. Phosphorylation of Thr45 in histone H3 is sensitive to Snail1 and Akt2 cellular levels; moreover, Snail1 upregulates the binding of phosphoThr45 histone H3 to the CDH1 promoter. These results uncover an unexpected role of Akt2 in transcriptional control and point out to phosphorylation of Thr45 in histone H3 as a new epigenetic mark related to Snail1 and Akt2 action.Oncogene advance online publication |
Description: | 12 p.-7 fig. |
Publisher version (URL): | http://dx.doi.org/ 10.1038/onc.2011.562 |
URI: | http://hdl.handle.net/10261/50517 |
DOI: | 10.1038/onc.2011.562 |
ISSN: | 0950-9232 |
E-ISSN: | 1476-5594 |
Appears in Collections: | (CIB) Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | ![]() View/Open |
Show full item record
Review this work
Review this work
Related articles:
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.