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Title

Differential cleavage of eIF4GI and eIF4GII in mammalian cells. Effects on translation

AuthorsCastelló, Alfredo; Álvarez, Enrique ; Carrasco Llamas, Luis CSIC ORCID
KeywordsVaccinia virus T7
Encephalomyocarditis virus
Issue Date7-Sep-2006
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Biological Chemistry 281(44): 33206-33216 (2006)
AbstractTwo isoforms of the translation initiation factor eIF4G, eIF4GI and eIF4GII, have been described in eukaryotic cells. The exact function of each isoform during the initiation of protein synthesis is still under investigation. We have developed an efficient and reliable method of expressing poliovirus 2Apro, which differentially proteolyzes eIF4GI and eIF4GII in a time- and dose-dependent manner. This system is based on the electroporation of an in vitro transcribed mRNA that contains the encephalomyocarditis virus internal ribosome entry site followed by the sequence of poliovirus 2Apro. In contrast to HeLa cells, expression of this protease in BHK-21 cells induces delayed hydrolysis kinetics of eIF4GI with respect to eIF4GII. Moreover, under these conditions the polyadenylate binding protein is not cleaved. Interestingly, translation of de novo synthesized luciferase mRNA is highly dependent on eIF4GI integrity, whereas ongoing translation is inhibited at the same time as eIF4GII cleavage. Moreover, reinitiation of a preexisting mRNA translation after polysome run-off is dependent on the integrity of eIF4GII. Notably, de novo translation of heat shock protein 70 mRNA depends little on eIF4GI integrity but is more susceptible to eIF4GII hydrolysis. Finally, translation of an mRNA containing encephalomyocarditis virus internal ribosome entry site when the two isoforms of eIF4G are differentially hydrolyzed has been examined
DescriptionEl pdf del artículo es la versión post-print.-- Article available at http://www.jbc.org/cgi/content/abstract/M604340200v1
Publisher version (URL)http://dx.doi.org/10.1074/jbc.M604340200
URIhttp://hdl.handle.net/10261/5043
DOI10.1074/jbc.M604340200
ISSN0021-9258
E-ISSN1083-351X
Appears in Collections:(CNB) Artículos
(CBM) Artículos

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