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Title

Sem1 is a functional component of the nuclear pore complex-associated messenger RNA export machinery

AuthorsBoulos Faza, Marius; Kemmler, Stefan; Jimeno, Sonia ; González-Aguilera, Cristina ; Aguilera, Andrés ; Hurt, Ed; Govind Panse, Vikram
Issue Date16-Mar-2009
PublisherRockefeller University Press
CitationJournal of Cell Biology 184(6): 833-846 (2009)
AbstractThe evolutionarily conserved protein Sem1/Dss1 is a subunit of the regulatory particle (RP) of the proteasome, and, in mammalian cells, binds the tumor suppressor protein BRCA2. Here, we describe a new function for yeast Sem1. We show that sem1 mutants are impaired in messenger RNA (mRNA) export and transcription elongation, and induce strong transcription-associated hyper-recombination phenotypes. Importantly, Sem1, independent of the RP, is functionally linked to the mRNA export pathway. Biochemical analyses revealed that, in addition to the RP, Sem1 coenriches with components of two other multisubunit complexes: the nuclear pore complex (NPC)-associated TREX-2 complex that is required for transcription-coupled mRNA export, and the COP9 signalosome, which is involved in deneddylation. Notably, targeting of Thp1, a TREX-2 component, to the NPC is perturbed in a sem1 mutant. These findings reveal an unexpected nonproteasomal function of Sem1 in mRNA export and in prevention of transcription-associated genome instability. Thus, Sem1 is a versatile protein that might stabilize multiple protein complexes involved in diverse pathways.
DescriptionContiene material suplementario.
URIhttp://hdl.handle.net/10261/50358
DOI10.1083/jcb.200810059
E-ISSN1540-8140
Identifiersdoi: 10.1083/jcb.200810059
issn: 0021-9525
Appears in Collections:(CABIMER) Artículos
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