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| Título: | An inverted repeat in the Tn21 integron determines the production of stem-loop DNA |
Autor: | Francia, M. Victoria; García-Lobo, Juan M. CSIC ORCID | Fecha de publicación: | 1996 | Editor: | Elsevier | Citación: | Plasmid 35(1): 31-36 (1996) | Resumen: | A 580-bp-long stem-loop DNA (sl-DNA) molecule was produced by a recombinant plasmid containing complete int and aadA genes from the transposon Tn21 cloned in the vector pUC18. A 50-bp-long inverted repeat found near the int gene stop codon could be involved in the generation of the sl-DNA molecule. Cloning of the dhfrII gene between the plasmid replication origin and this IR produced an extended sl-DNA including the dhfrII gene. Tn21 recombination hot spot 2 was also able to direct the production of sl-DNA. © 1996 Academic Press, Inc. | URI: | http://hdl.handle.net/10261/50347 | DOI: | 10.1006/plas.1996.0003 | Identificadores: | doi: 10.1006/plas.1996.0003 issn: 0147-619X |
| Aparece en las colecciones: | (IBBTEC) Artículos |
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| accesoRestringido.pdf | 15,38 kB | Adobe PDF |  Visualizar/Abrir |
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