Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/50315
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Casal, Enriqueta | - |
dc.contributor.author | Lebrón-Aguilar, Rosa | - |
dc.contributor.author | Moreno, F. Javier | - |
dc.contributor.author | Corzo, Nieves | - |
dc.contributor.author | Quintanilla-López, Jesús Eduardo | - |
dc.date.accessioned | 2012-05-29T09:09:41Z | - |
dc.date.available | 2012-05-29T09:09:41Z | - |
dc.date.issued | 2010-04 | - |
dc.identifier.citation | Rapid Communications in Mass Spectrometry 24(7): 885-893 (2010) | es_ES |
dc.identifier.issn | 0951-4198 | - |
dc.identifier.uri | http://hdl.handle.net/10261/50315 | - |
dc.description | 9 páginas, 6 figuras, 2 esquemas.-- El pdf del artículo es la versión de autor. | es_ES |
dc.description.abstract | Sialylated O-linked oligosaccharides are involved in many biological processes, such as cell-cell interactions, cell-substance adhesion, and virus-host interactions. These activities depend on their structure, which is frequently determined by tandem mass spectrometry. However, these spectra are frequently analyzer-dependent, which makes it difficult to develop widely applicable analytical methods. In order to deepen the origin of this behavior, two couples of isomers of sialylated O-linked oligosaccharides, NeuAcα2-3Galβ1-3GalNAc-ol/Galβ1-3(NeuAcα2-6)GalNAc-ol and NeuGcα2-3Galβ1-3GalNAc-ol/Galβ1-3(NeuGcα2-6)GalNAc-ol, were analyzed by liquid chromatography/negative electrospray ionization ion trap tandem mass spectrometry (LC/ESI(−)-MSn) using both an ion trap and a triple quadrupole mass spectrometer. Results clearly showed that while ions obtained in the triple quadrupole instrument fitted very well with the standard fragmentation routes, in the ion trap several intense ions could not be explained by these rules, specially a fragment at m/z 597. Furthermore, this ion was observed in the mass spectrum of those isomers that sialic acid binds to GalNAc by an α2-6 linkage. From the MS3 spectrum of this ion an unexpected structure was deduced, and it led to propose alternative fragmentation pathways. Molecular mechanics calculations suggested that the found atypical route could be promoted by a hydrogen bond located only in α2-6-linked oligosaccharides. It has also been demonstrated that this process follows a slow kinetic, explaining why it cannot be observed using an ion beam-type mass analyzer. In conclusion, ion traps seem to be more appropriate than triple quadrupoles to develop a reliable analytical method to distinguish between isomeric O-linked glycans. | es_ES |
dc.description.sponsorship | Financial support was obtained from projects ANALISYC S-0505/ AGR/000312 from the Comunidad de Madrid, Consolider Ingenio 2010 (FUN-C-FOOD); CSD 2007-00063 from the Ministerio de Educación y Ciencia; and PIF-SIALOBIOTIC 200870F010-1 and -2 from CSIC. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley-Blackwell | es_ES |
dc.relation.isversionof | Postprint | - |
dc.rights | openAccess | es_ES |
dc.title | Selective linkage detection of O-sialoglycan isomers by negative electrospray ionization ion trap tandem mass spectrometry | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1002/rcm.4463 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1002/rcm.4463 | es_ES |
dc.identifier.e-issn | 1097-0231 | - |
dc.contributor.funder | Comunidad de Madrid | - |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (IFI) Artículos (IQF) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Selective linkage detection of O-sialoglycan.pdf | 1,39 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
7
checked on 12-abr-2024
WEB OF SCIENCETM
Citations
7
checked on 22-feb-2024
Page view(s)
347
checked on 22-abr-2024
Download(s)
315
checked on 22-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.