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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/50283
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dc.contributor.authorNavascués, Joaquín-
dc.contributor.authorLeón, Javier-
dc.contributor.authorBerciano, María T.-
dc.contributor.authorLafarga, Miguel-
dc.contributor.authorDelgado, M. Dolores-
dc.date.accessioned2012-05-29T07:10:19Z-
dc.date.available2012-05-29T07:10:19Z-
dc.date.issued2006-
dc.identifierdoi: 10.1242/jcs.02890-
dc.identifierissn: 0021-9533-
dc.identifier.citationJournal of Cell Science 119(9): 1746-1759 (2006)-
dc.identifier.urihttp://hdl.handle.net/10261/50283-
dc.descriptionet al.-
dc.description.abstractMultiple functions have been reported for the transcription factor and candidate tumour suppressor, CTCF. Among others, they include regulation of cell growth, differentiation and apoptosis, enhancer-blocking activity and control of imprinted genes. CTCF is usually localized in the nucleus and its subcellular distribution during the cell cycle is dynamic; CTCF was found associated with mitotic chromosomes and the midbody, suggesting different roles for CTCF at different stages of the cell cycle. Here we report the nucleolar localization of CTCF in several experimental model systems. Translocation of CTCF from nucleoplasm to the nucleolus was observed after differentiation of K562 myeloid cells and induction of apoptosis in MCF7 breast cancer cells. CTCF was also found in the nucleoli in terminally differentiated rat trigeminal ganglion neurons. Thus our data show that nucleolar localization of CTCF is associated with growth arrest. Interestingly, the 180 kDa poly(ADP-ribosyl)ated isoform of CTCF was predominantly found in the nucleoli fractions. By transfecting different CTCF deletion constructs into cell lines of different origin we demonstrate that the central zinc-finger domain of CTCF is the region responsible for nucleolar targeting. Analysis of subnucleolar localization of CTCF revealed that it is distributed homogeneously in both dense fibrillar and granular components of the nucleolus, but is not associated with fibrillar centres. RNA polymerase I transcription and protein synthesis were required to sustain nucleolar localization of CTCF. Notably, the labelling of active transcription sites by in situ run-on assays demonstrated that CTCF inhibits nucleolar transcription through a poly(ADP-ribosyl)ation-dependent mechanism.-
dc.description.sponsorshipV.T. is supported by a PhD fellowship from Fondo Investigación Sanitaria (FIS) and from the University of Cantabria. This work was supported by grants FIS04/1083 from the Spanish Ministerio de Sanidad y Consumo (M.D.D.), BFI2002-0454 and BFU2005-01030 from Dirección General de Investigación Científica (M.L., M.T.B.), SAF02-4193 from the Spanish Ministerio de Educación (J.L.), a Biomedical Research Collaboration Grant from the Wellcome Trust (E.K., M.D.D., J.L.), the Breast Cancer Campaign (F.D., E.K.), the Association for International Cancer Research (I.C., E.K.), a Research Promotion Fund from the University of Essex (F.D., I.C., E.K.) and a PhD studentship from the University of Essex (D.F.).-
dc.language.isoeng-
dc.publisherCompany of Biologists-
dc.rightsopenAccess-
dc.titleTargeting of CTCF to the nucleolus inhibits nucleolar transcription through a poly(ADP-ribosyl)ation-dependent mechanism-
dc.typeartículo-
dc.identifier.doi10.1242/jcs.02890-
dc.relation.publisherversionhttp://dx.doi.org/10.1242/jcs.02890-
dc.date.updated2012-05-29T07:10:19Z-
dc.description.versionPeer Reviewed-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.languageiso639-1en-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairetypeartículo-
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