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Título

Quantitative estimation of plum pox virus targets acquired and transmitted by a single Myzus persicae

AutorMoreno, Aránzazu ; Fereres, Alberto ; Cambra, Mariano
Palabras claveMyzus persicae
PPV
Virology
RT-PCR
RNA
Fecha de publicación2009
EditorSpringer
CitaciónArchives of Virology 154: 1391-1399 (2009)
ResumenThe viral charge acquired and inoculated by single aphids in a non-circulative transmission is estimated using plum pox virus (PPV). A combination of electrical penetration graph and TaqMan real-time RT-PCR techniques was used to establish the average number of PPV RNA targets inoculated by an aphid in a single probe (26,750), approximately half of the acquired ones. This number of PPV targets is responsible for a systemic infection of 20% on the inoculated receptor plants. No significant differences were found between the number of PPV RNA targets acquired after one and after five intracellular punctures (pd), but the frequency of infected receptor plants was higher after 5 pd. The percentage of PPV-positive leaf discs after just 1 pd of inoculation probe (28%/4,603 targets) was lower than after 5 pd (45.8%/135 9 106 targets). The methodology employed could be easily extended to other virus–vector–host combinations to improve the accuracy of models used in virus epidemiology.
Descripción9 páginas, ilustraciones y tablas estadísticas
Versión del editorhttp://dx.doi.org/10.1007/s00705-009-0450-5
URIhttp://hdl.handle.net/10261/50279
DOI10.1007/s00705-009-0450-5
ISSN0304-8608
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