English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/50137
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Selective adsorption of small proteins on large-pore anion exchangers coated with medium size proteins

AuthorsBolívar Bolívar, Juan Manuel ; Batalla Bosquet, Pilar ; Mateo González, César ; Carrascosa, Alfonso V. ; Pessela, Benevides C. ; Guisán, José Manuel
Issue Date2010
PublisherElsevier
CitationColloids and Surfaces B: Biointerfaces 78(1): 140-145 (2010)
AbstractA new anion exchanger support has been designed for the selective adsorption of small proteins. This has been achieved activating an aminated support with glutaraldehyde and further coating the support surface with bovine serum albumin (BSA). In this support, " wells" are generated by two neighborhoods BSA molecules, on the bottom of those " wells" glutaraldehyde groups are exposed out ready to react with small molecules that have a size small enough to be accommodated between two BSA molecules on the pre-existing support. However, the BSA surface was not inert enough adsorbing many proteins, thereby reducing the selectivity of the system. A further solution was coating the immobilized BSA molecules with dextran, reducing the adsorption of protein on the BSA surface. This new matrix has been evaluated in the selective adsorption of the very small beta-lactoglobulins and alpha-lactalbumin from dairy whey, achieving the selective adsorption of both small proteins while other larger proteins from dairy whey remained in the supernatant. Moreover, a protein crude extract has been offered to the new matrix, and only small proteins could be adsorbed on the support (as probed by gel filtration).Thus this amino-glutaraldehyde-BSA-dextran-Sepharose is a matrix that may be used to selectively ionically adsorb proteins that were smaller than BSA (62. kDa). This strategy may be used for any other kind of adsorbing groups (chelating agents, boronic acid, etc.), or using proteins with different sizes to coat the support, designing tailor-made supports that may permit the fractioning of proteins following their sizes and by adsorption/desorption on different matrices. © 2010 Elsevier B.V.
URIhttp://hdl.handle.net/10261/50137
DOI10.1016/j.colsurfb.2010.02.030
Identifiersdoi: 10.1016/j.colsurfb.2010.02.030
issn: 0927-7765
Appears in Collections:(ICP) Artículos
(IFI) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.