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Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids

AutorRevilla, Carlos ; Garcillán-Barcia, M. Pilar; Fernández-López, Raul; Thomson, Nicholas R.; Sanders, Mandy; Cheung, Martin; Thomas, Christopher M.; Cruz, Fernando de la
Fecha de publicación2008
EditorAmerican Society for Microbiology
CitaciónAntimicrobial Agents and Chemotherapy 52(4): 1472-1480 (2008)
ResumenDNA sequence analysis of five IncW plasmids (R388, pSa, R7K, pIE321, and pIE522) demonstrated that they share a considerable portion of their genomes and allowed us to define the IncW backbone. Among these plasmids, the backbone is stable and seems to have diverged recently, since the overall identity among its members is higher than 95%. The only gene in which significant variation was observed was trwA; the changes in the coding sequence correlated with parallel changes in the corresponding TrwA binding sites at oriT, suggesting a functional connection between both sets of changes. The present IncW plasmid diversity is shaped by the acquisition of antibiotic resistance genes as a consequence of the pressure exerted by antibiotic usage. Sequence comparisons pinpointed the insertion events that differentiated the five plasmids analyzed. Of greatest interest is that a single acquisition of a class I integron platform, into which different gene cassettes were later incorporated, gave rise to plasmids R388, pIE522, and pSa, while plasmids R7K and pIE321 do not contain the integron platform and arose in the antibiotic world because of the insertion of several antibiotic resistance transposons. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Versión del editorhttp://dx.doi.org/10.1128/AAC.00982-07
URIhttp://hdl.handle.net/10261/50113
DOI10.1128/AAC.00982-07
Identificadoresdoi: 10.1128/AAC.00982-07
issn: 0066-4804
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