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Title

SUMO-1 transiently localizes to Cajal bodies in mammalian neurons

AuthorsNavascués, Joaquín; Bengoechea, Rocío; Tapia, Olga; Casafont, Íñigo; Berciano, María T.; Lafarga, Miguel
Issue Date2008
PublisherElsevier
CitationJournal of Structural Biology 163: 137-146 (2008)
AbstractCajal bodies (CBs) are nuclear organelles involved in the maturation of small nuclear ribonucleoproteins required for the processing of pre-mRNAs. They concentrate coilin, splicing factors and the survival of motor neuron protein (SMN). By using immunocytochemistry and transfection experiments with GFP-SUMO-1, DsRed1-Ubc9, GFP-coilin and GFP-SMN constructs we demonstrate the presence of SUMO-1 and the SUMO conjugating enzyme (Ubc9) in a subset of CBs in undifferentiated neuron-like UR61 cells. Furthermore, SUMO-1 is transiently localized into neuronal CBs from adult nervous tissue in response to osmotic stress or inhibition of methyltransferase activity. SUMO-1-positive CBs contain coilin, SMN and small nuclear ribonucleoproteins, suggesting that they are functional CBs involved in pre-mRNA processing. Since coilin and SMN have several putative motifs of SUMO-1 modification, we suggest that the sumoylation of coilin and/or SMN might play a role in the molecular reorganization of CBs during the neuronal differentiation or stress-response. © 2008 Elsevier Inc. All rights reserved.
URIhttp://hdl.handle.net/10261/50106
DOI10.1016/j.jsb.2008.04.013
Identifiersdoi: 10.1016/j.jsb.2008.04.013
issn: 1047-8477
Appears in Collections:(IBBTEC) Artículos
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