Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/50026
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
DC FieldValueLanguage
dc.contributor.authorKlein, Melinda A.-
dc.contributor.authorLópez-Millán, Ana Flor-
dc.contributor.authorGrusak, Michael A.-
dc.date.accessioned2012-05-24T07:50:30Z-
dc.date.available2012-05-24T07:50:30Z-
dc.date.issued2012-02-
dc.identifier.citationKlein MA, López-Millán AF, Grusak MA. Quantitative trait locus analysis of root ferric reductase activity and leaf chlorosis in the model legume, Lotus japonicus. Plant and Soil 351 (1-2): 363-376 (2012)es_ES
dc.identifier.issn0032-079X-
dc.identifier.urihttp://hdl.handle.net/10261/50026-
dc.description14 Pags., 3 Tabls., 6 Figs.es_ES
dc.description.abstractBackground and aims Ferric reductase activity is a rate-limiting step in the accumulation of iron by Strategy I plants. Preliminary work with Lotus japonicus accessions Miyakojima MG-20 and Gifu B-129 identified differences in shoot chlorosis and ferric reductase activity. This study assessed the genetic basis for these differences. Methods Lines of a recombinant inbred population, derived from Miyakojima and Gifu, were tested for whole-root ferric reductase activity and shoot chlorosis following iron-limited growth. A ferric reductase gene (LjFRO1) was cloned from both parents. Protein sequence analysis, transcript abundance, and yeast complementation studies were conducted with the two parental alleles. Results A single quantitative trait locus (QTL) was identified for both ferric reductase activity and shoot chlorosis, with each QTL explaining ~30% of the variation and both overlapping across the same region of chromosome 3. LjFRO1 mapped to chromosome 3, but to a region adjacent to the reductase and chlorosis loci. Nucleotide variation in LjFRO1 parental alleles was identified, as were minor functional differences between the two proteins. Conclusions The results indicate that both allelic variation (providing potential functional differences) and unidentified molecular components (derived from non-LjFRO1 genetic loci) can contribute to the regulation of ferric reductase activity and chlorosis susceptibility.es_ES
dc.description.sponsorshipThis work was funded in part by funds from USDA-ARS under agreement No. 58-6250-0-008 to M.A.G.es_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.rightsopenAccesses_ES
dc.subjectChlorosises_ES
dc.subjectFerric reductasees_ES
dc.subjectIron nutritiones_ES
dc.subjectLotus japonicuses_ES
dc.subjectQTLes_ES
dc.titleQuantitative trait locus analysis of root ferric reductase activity and leaf chlorosis in the model legume, Lotus japonicuses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1007/s11104-011-0972-y-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1007/s11104-011-0972-yes_ES
dc.identifier.e-issn1573-5036-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairetypeartículo-
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:(EEAD) Artículos
Files in This Item:
File Description SizeFormat
mod vers.aut pend.pdf20,83 kBAdobe PDFThumbnail
View/Open
Show simple item record

Google ScholarTM

Check

Altmetric

Dimensions


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.