English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/49981
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Caveolar domain organization and trafficking is regulated by Abl kinases and mDia1

AutorEcharri, Asier; Muriel, Olivia; Pavón, Dácil M.; Azegrouz, Hind; Escolar, Fernando; Terrón, María Carmen ; Sánchez-Cabo, Fátima; Martínez, Fernando ; Montoya, María C.; Llorca, Óscar ; Pozo, Miguel A. del
Palabras claveVesicle trafficking
actin cytoskeleton
cell adhesion
Abl tyrosine kinases
caveolin-1
Fecha de publicación27-mar-2012
EditorCompany of Biologists
CitaciónJournal of Cell Science Mar 27(2012)
ResumenCaveolin-1 (Cav1)/caveolae biology is intimately linked to actin dynamics and adhesion receptors. Caveolar domains are organized in hierarchical levels of complexity from curved or flatten caveolae to large, higher-order caveolar rosettes. We report that stress fibers controlled by Abl kinases and mDia1 determine the level of caveolar domain organization, which conditions the subsequent inward trafficking of caveolar domains induced upon loss of cell adhesion from the extracellular matrix. Abl-deficient cells show decreased content of stress fibers, a smaller stress-fiber co-aligned Cav1 pool and increased clustering of Cav1/caveolae at the cell surface. Defective caveolar linkage to stress fibers prevents the formation of big caveolar rosettes upon loss of cell adhesion, correlating with a lack of inward trafficking. Live imaging of stress fibers and Cav1 showed that the actin-linked Cav1 pool loses its spatial organization in the absence of actin polymerization and is dragged and clustered by depolymerizing filaments. We identify mDia1 as the actin polymerization regulator downstream of Abl kinases that controls the stress fiber-linked Cav1 pool. mDia1 knockdown results in Cav1/caveolae clustering and defective inward trafficking upon loss of cell adhesion. In contrast, cell elongation imposed by the excess of stress fibers induced by active mDia1 flattens caveolae. Furthermore, active mDia1 rescues the actin co-aligned Cav1 pool and Cav1 inward trafficking upon loss of adhesion in Abl-deficient cells. Thus, caveolar domain organization and trafficking are tightly coupled to adhesive and stress fiber regulatory pathways
Descripción54 páginas, 8 figuras
Versión del editorhttp://dx.doi.org/ 10.1242/​jcs.090134
URIhttp://hdl.handle.net/10261/49981
DOI10.1242/​jcs.090134
ISSN0021-9533
E-ISSN1477-9137
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Acceso restringido.pdf21,67 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.