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| Title: | 2D gels and their third-dimension potential |
| Authors: | Schvartzman, Jorge Bernardo  ; Martínez-Robles, María Luisa ; López, Virginia; Hernandez, Pablo ; Krimer, Dora B. |
| Keywords: | DNA Replication Initiation Termination Barriers Fork reversal |
| Issue Date: | 23-Mar-2012 |
| Publisher: | Elsevier |
| Citation: | Methods (2012) |
| Abstract: | Two-dimensional (2D) agarose gel electrophoresis is one of the most powerful methods to analyze the mass and shape of replication intermediates. It is often use to map replication origins but it is also useful to characterize termination of replication, replication fork barriers and even replication fork reversal. Here, we present protocols, figures and movies with a thorough description of different modes of replication for linear DNA fragments and the corresponding patterns they generate in 2D gels |
| Description: | 159 páginas, 9 figuras, 23 videos |
| Publisher version (URL): | http://dx.doi.org/ 10.1016/j.ymeth.2012.03.013 |
| URI: | http://hdl.handle.net/10261/49952 |
| DOI: | 10.1016/j.ymeth.2012.03.013 |
| ISSN: | 1046-2023 |
| E-ISSN: | 1095-9130) |
| Appears in Collections: | (CIB) Artículos |
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