English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/49918
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

The cryo-EM structure of the UPF-EJC complex shows UPF1 poised toward the RNA 3´ end.

AuthorsMelero, Roberto ; Buchwald, G.; Castaño, Raquel; Raabe, M.; Gil, David; Lázaro, M.; Urlaub, H.; Conti, E.; Llorca, Óscar
Issue Date22-Apr-2012
PublisherNature Publishing Group
CitationNature Structural & Molecular Biology, 19 (4) : 498-505 (2012)
AbstractNonsense-mediated mRNA decay (NMD) is a eukaryotic surveillance pathway that degrades aberrant mRNAs containing premature termination codons (PTCs). NMD is triggered upon the assembly of the UPF surveillance complex near a PTC. In humans, UPF assembly is prompted by the exon junction complex (EJC). We investigated the molecular architecture of the human UPF complex bound to the EJC by cryo-EM and using positional restraints from additional EM, MS and biochemical interaction data. The heptameric assembly is built around UPF2, a scaffold protein with a ring structure that closes around the CH domain of UPF1, keeping the helicase region in an accessible and unwinding-competent state. UPF2 also positions UPF3 to interact with the EJC. The geometry is such that this transient complex poises UPF1 to elicit helicase activity toward the 3′ end of the mRNP
Description8 páginas, 7 figuras -- PAGS nros. 498-505
Publisher version (URL)http://dx.doi.org/ 10.1038/nsmb.2287
URIhttp://hdl.handle.net/10261/49918
DOI10.1038/nsmb.2287
ISSN1545-9993
E-ISSN1545-9985
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
Molero et al_Nat_Struct & Mol Biology_2012.pdf36,28 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.