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Título: | RNA aptamer-mediated interference of HCV replication by targeting the CRE-5BSL3.2 domain |
Autor: | Marton, S.; Romero-López, C.; Berzal-Herranz, Alfredo |
Palabras clave: | HCV genome CRE RNA structure-function RNA Aptamers |
Fecha de publicación: | 21-may-2012 |
Resumen: | The RNA genome of hepatitis C virus (HCV) contains multiple conserved structural RNA domains that play key roles in essential viral processes. A conserved structural component within the 3’ end of the region coding for viral RNA-dependent RNA polymerase (NS5B) has been characterized as a functional cis-acting replication element (CRE). This paper reports the ability of two RNA aptamers, P-58 and P-78, to interfere with HCV replication by targeting the essential 5BSL3.2 domain within this CRE. Structure probing assays showed the binding of the aptamers to the CRE to result in a structural reorganization of the apical portion of the 5BSL3.2 stem-loop domain. This interfered with the binding of the NS5B protein to the CRE and induced a significant reduction in HCV replication (≈50%) in an autonomous subgenomic HCV replication system. These results highlight the potential of this CRE as a target for the development of anti-HCV therapies, and underscore the potential of antiviral agents based on RNA aptamer molecules. |
Descripción: | 32 páginas,ilustraciones.--Texto completo borrador del autor. |
URI: | http://hdl.handle.net/10261/49917 |
Aparece en las colecciones: | (IPBLN) Artículos |
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Fichero | Descripción | Tamaño | Formato | |
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RNA aptamer2.pdf | 377,71 kB | Adobe PDF | ![]() Visualizar/Abrir |
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